Expression and role of triggering receptor expressed on myeloid cells 2 in high glucose-treated microglia
10.16352/j.issn.1001-6325.2024.02.0167
- VernacularTitle:髓系细胞触发受体2在高糖处理的小胶质细胞中的表达及作用
- Author:
Zhaohui WANG
1
;
Xiao LIU
;
Yue ZHOU
;
Xinyi WEI
;
Yue WANG
;
Junfa LI
;
Li ZHAO
Author Information
1. 首都医科大学 基础医学院 神经生物学系,北京 100006
- Keywords:
high glucose;
microglia;
myeloid cell trigger receptor 2(TREM2)
- From:
Basic & Clinical Medicine
2024;44(2):167-173
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of triggering receptor expressed on myeloid cells 2(TREM2)in high-glucose microglia and to investigate the role of TREM2 in the proliferation,migration and phagocytosis of high-glucose microglia.Methods Microglia cells were divided into control group and high-glucose treatment group(67.5 mmol/L glucose,24 h).The microglia cells were counted and the expression of Iba1 and TREM2 was de-tected.TREM2 siRNA was transfected to detect the proliferation and migration of microglia.The amyloid β-peptide(Aβ)with a fluorescent tag was added to observe the phagocytosis of Aβ by microglia.Results Compared to normal microglia,the number of microglia significantly decreased after high-glucose treatment(P＜0.001),while the ex-pression of TREM2 and Iba1 markedly increased(P＜0.001).High glucose and TREM2 did not affect the prolifer-ation of microglia.Compared to the normal group,the migration of microglia significantly decreased after high-glu-cose treatment(P＜0.05)and TREM2 did not affect the migration ability of high-glucose microglia.Compared to the normal group,the phagocytosis of Aβ by microglia significantly decreased in the high-glucose treated group(P＜0.001).Furthermore,TREM2 knockdown further decreased the phagocytosis of Aβ by high-glucose microglia(P＜0.001).Conclusions The expression of TREM2 in microglia significantly increases after high-glucose treat-ment,which significantly affects phagocytosis of Aβ by microglia.
