Nutlin-3a regulates mouse adipose function by inhibition of CIDEC expression
10.16352/j.issn.1001-6325.2024.02.0154
- VernacularTitle:Nutlin-3a通过抑制CIDEC的表达调控小鼠脂肪功能
- Author:
Enhui CHEN
1
,
2
;
Jiahui YANG
;
Wei ZHAO
;
Xianghong XIE
;
Yanfang GUO
;
Xiaojun LIU
;
Li YAN
Author Information
1. 中国医学科学院基础医学研究所 北京协和医学院基础学院 病理生理学系 北京 100005
2. 中国医学科学院基础医学研究所 北京协和医学院基础学院 重大疾病共性机制研究全国重点实验室,北京 100005
- Keywords:
Nutlin-3a;
fat metabolism;
adipose tissue;
CIDEC
- From:
Basic & Clinical Medicine
2024;44(2):154-158
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Nutlin-3a,a mouse double minute 2 homolog(MDM2)inhibitor,on lipid metabolism of mouse adipose.Methods High-fat diet-induced obesity(DIO)C57BL/6J mice were randomly divided into a control group injected with DMSO and an experimental group injected with Nutlin-3a.Then we conducted glucose tolerance(GTT)and insulin tolerance(ITT)tests.The epididymal white adipose tissue(eWAT),inguinal white adipose tissue(iWAT)and brown adipose tissue(BAT)of animals were isolated and microscopy of WATs with hematoxylin-eosin(HE)staining was performed to observe the morphological changes of adipocytes.The expression of lipid metabolism related gene cell death-inducing DFF45-like effector C(CIDEC)in eWAT were detected by qPCR and Western blot.Results Compared with the control group,Nutlin-3a was found to promote the body weight(P＜0.001),but no effect on glucose tolerance and insulin sensitivity in DIO mice.Nutlin-3a treatment decreased the size of adipocytes and fat deposition in adipose tissue and downregulated the mRNA and protein levels of CIDEC in eWAT.Conclusions Nutlin-3a inhibits the formation of lipid droplets by downregulating expression of CIDEC in white adipose tissue.
