Marein alleviates myocardial fibrosis in diabetic mice
10.16352/j.issn.1001-6325.2024.01.0051
- VernacularTitle:马里苷减轻糖尿病小鼠心肌纤维化
- Author:
Yating TIAN
1
,
2
;
Fang ZHANG
;
Boxiang ZHANG
;
Tian LI
Author Information
1. 新疆医科大学 基础医学院 组织学胚胎学教研室
2. 新疆地方病分子生物学重点实验室, 新疆 乌鲁木齐 830011
- Keywords:
marein;
diabeties;
fibrosis;
transforming growth factor-β1(TGF-β1)
- From:
Basic & Clinical Medicine
2024;44(1):51-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of marein on myocardial fibrosis in diabetic mice.Methods Ten lep-tin receptor gene defective heterozygous(db/m)mice aged 5-6 weeks were selected as the control group and 30 diabetic mice with leptin receptor gene defective db/db were divided into:db/db group(db/db,n=10),metformin(Met)positive group(280 mg/kg daily,n=10)and marein drug intervention group(50 mg/kg,n=10).After continuous administration for 8 weeks,the cardiac morphological changes were observed by HE staining and Masson staining.The distribution and expression of vimentin were detected by immunohistochemis-try method.The expression of fibronectin,vimentin,and transforming growth factor-β1(TGF-β1)protein in cardiac tissue was detected by Western blot.Results Myocardial fiber hypertrophy was observed in db/db group,and myocardial structural damage was improved in metformin group and marein group.Compared with db/m group,the expression of myocardial collagen fiber in db/db group increased(P＜0.01),while the expression of myo-cardial collagen fiber in metformin group and marein group decreased(P＜0.01).Compared with the control group,the expression of vimentin in myocardial tissue of db/db group was significantly increased(P＜0.01),while the expression of vimentin in metformin group and marein group was significantly decreased(P＜0.01).The expression of fibronectin,vimentin and TGF-β1 in db/db group was significantly increased as compared with those in db/m group(P＜0.01),while the expression of fibronectin,vimentin and TGF-β1 in metformin group and marein group were significantly decreased(P＜0.01).Conclusions Marein improves myocardial fibrosis in diabetic db/db mice.
