Alpinetin alleviates bleomycin-induced pulmonary fibrosis in mice
10.16352/j.issn.1001-6325.2023.12.1827
- VernacularTitle:山姜素减轻博来霉素诱导的小鼠肺纤维化
- Author:
Changxing SI
1
;
Yanyan DING
;
Fengxian YIN
Author Information
1. 北京市大兴区人民医院 呼吸与危重症医学科,北京 102600
- Keywords:
alpinetin;
bleomycin;
endoplasmic reticulum stress;
pulmonary fibrosis
- From:
Basic & Clinical Medicine
2023;43(12):1827-1833
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and potential mechanism of alpinetin(ALPN)on bleomycin(BLM)-induced pulmonary fibrosis in mice.Methods The mice were randomly divided into control group,model group(intratracheally instilled BLM),low-dose(L-ALPN group)and high-dose ALPN groups(H-ALPN group)(10 mg/kg or 30 mg/kg ALPN daily,respectively)with 10 in each.Lung tissues were collected,and the alveolar structure and pathological morphology were microscopied by HE and Masson staining;mRNA and protein expres-sions of collagen Ⅰ,TGF-β1,E-cadherin,α-SMA,p-PERK,PERK,CHOP and GRP78 in lung tissue were de-tected by RT-qPCR,immunohistochemistry and Western blot,respectively.Results Compared with control group,the lung of the model group showed fibrotic changes,and the expression of collagenⅠ,TGF-β1,α-SMA,p-PERK/PERK,CHOP and GRP78 in lung tissue was significantly increased(P＜0.01).E-cadherin expression was significantly decreased(P＜0.01).Compared with model group,pulmonary fibrosis was significantly alleviated in low and high doses ALPN groups,the expression of collagenⅠ,TGF-β1,α-SMA,p-PERK/PERK,CHOP and GRP78 in lung tissue were significantly decreased(P＜0.05 or P＜0.01),and the expressionof E-cadherin was sig-nificantly increased(P＜0.05 or P＜0.01).Conclusions ALPN may alleviate BLM-induced pulmonary fibrosis,and this effect may be attributed to the inhibition of endoplasmic reticulum stress.
