Esketamine reduces ischemia-reperfusion injury of skeletal muscle of rats
10.16352/j.issn.1001-6325.2023.12.1822
- VernacularTitle:艾司氯胺酮减轻大鼠骨骼肌缺血再灌注损伤
- Author:
Peigen YUAN
1
;
Shunli CHEN
;
Yuanlu SHAN
;
Binbin XUE
;
Yuzhu YE
;
Lina LIN
Author Information
1. 温州医科大学附属第一医院 麻醉科,浙江 温州 325000
- Keywords:
esketamine;
ischemia-reperfusion injury;
oxidative stress;
Nrf2
- From:
Basic & Clinical Medicine
2023;43(12):1822-1826
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of low dose esketamine in pretreated limb ischemia-reperfusion inju-ry.Methods The rats were divided into sham-operated group(Sham group),ischemia-reperfusion group(I/R group,3 h of ischemia and 2 h of reperfusion),esketamine group(ESK group,ip,5 mg/kg).The plasma con-centrations of creatine kinase(CK)and lactate dehydrogenase(LDH)were measured.The wet/dry weight ratio of skeletal muscle was immediately detected.The gastrocnemius muscle was harvested and the level of malondial-dehyde(MDA)and superoxide dismutase(SOD)was detected by colorimetric assay.The expression of nuclear factor erythroid 2-related factor 2(Nrf2)and heme oxygenase-1(HO-1)were detected by Western blot and im-munohistochemical staining.Results Compared with Sham group,the index of wet/dry weight ratio,MDA,CK,LDH,Nrf2 and HO-1 were all increased,but SOD was decreased in I/R group(P＜0.05).Compared with I/R group,the index of wet/dry weight ratio,MDA,CK and LDH were significantly lower,but SOD and Nrf2 and HO-1 were significantly higher in ESK group(P＜0.05).Conclusions ESK may increase Nrf2 in the nucleus,thereby increase the HO-1 protein as an antioxidant agent.
