Over-expression of APMAP alleviates glomerular podocyte injury in adriamycin nephropathy
10.16352/j.issn.1001-6325.2023.12.1814
- VernacularTitle:过表达APMAP减轻阿霉素肾病肾小球足细胞损伤
- Author:
Jingyi WU
1
;
Wenjing FAN
;
Donghua YAN
Author Information
1. 江苏盛泽医院 儿科,江苏 苏州 215228
- Keywords:
adriamycin nephropathy;
glomerular podocytes;
adipocyte plasma membrane-associated protein(APMAP);
NF-κB signa-ling pathway
- From:
Basic & Clinical Medicine
2023;43(12):1814-1821
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of adipocyte plasma membrane-associated protein(APMAP)over-expression on glomerular podocyte injury in adriamycin(ADR)nephropathy.Methods The rat model of adriamy-cin nephropathy was constructed by tail vein injection of adriamycin,the expression of APMAP and NF-κB p65 in renal tissue was measured by immunohistochemistry.A mouse glomerular podocytes MPC-5 cell line with APMAP gene over-expression was constructed,then podocyocytes injury model was induced by 0.5 μmol/L ADR and trea-ted with NF-κB signaling pathway activator CU-T12-9.The proliferation of cells was checked by CCK-8.The activity of lactate dehydrogenase(LDH)was determined by ELISA.The apoptosis of podocytes was determined by flow cytometry.Western blot was used to detect protein expressions of NF-κB p65,p-NF-κB p65 and TNF-α.Results APMAP was expressed in kidney tissue of doxorubicin nephropathy rats at a low level,while NF-κB p65 was significantly high expressed(P＜0.05).Over-expression of APMAP increased proliferation of MPC-5 cells and decreased LDH activity,apoptosis rate,and also down-regulated protein expression of NF-κB p65,P-NF-κB p65 and TNF-α under ADR exposure(P＜0.05).However,combined treatment with CU-T12-9 significantly inhibited the ameliorative effect of APMAP over-expression on the damage of MPC-5 cells exposed to ADR.Conclusions The over-expression of APMAP can inhibit ADR-induced glomerular podocyte injury,and its mechanism might be related to the inhibition of NF-κB signaling pathway.
