Regulatory Mechanism of Drug-Containing Serum of Jinghou Zengzhi Prescription on GDF9 Expression and Apoptosis of Ovarian Granulosa Cells in Rats with Controlled Ovarian Hyperstimulation
10.13359/j.cnki.gzxbtcm.2024.03.031
- VernacularTitle:经后增殖方含药血清对超排卵大鼠卵巢颗粒细胞GDF9表达及凋亡的调控机制
- Author:
Zhen YANG
1
;
Xiao-Yan CHEN
;
Shao-Ru JIANG
;
Shu-Zhu YE
;
Xiao-Hong FANG
;
Wei-Min DENG
;
Xin-Yu GUO
Author Information
1. 揭阳市人民医院,广东揭阳 522000
- Keywords:
qi and blood replenishing method;
Jinghou Zengzhi Prescription;
controlled ovarian hyperstimulation(COH);
apoptosis;
growth differentiation factor 9(GDF9);
p38 mitogen-activated protein kinase(p38MAPK);
nuclear transcription factor κB(NF-κB);
granulosa cell
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(3):735-741
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the regulatory mechanism of drug-containing serum of Jinghou Zengzhi Prescription based on qi and blood replenishing method on the expression of growth and differentiation factor 9(GDF9)and apoptosis of ovarian granulosa cells in rats with controlled ovarian hyperstimulation(COH).Methods Serum of COH rats(blank serum)and serum of COH rats gavaged by the Jinghou Zengzhi Prescription were prepared.A COH rat model was established and ovarian granulosa cells were collected.The experiment was divided into 5 groups:blank serum group,drug-containing serum group,drug-containing serum+SB203580[p38 mitogen-activated protein kinase(p38MAPK)inhibitor]group,drug-containing serum + PDTC[nuclear transcription factor κB(NF-κB)inhibitor]group,drug-containing serum + SB203580 + PDTC group.The mRNA expression levels of p38MAPK,casein kinase 2(CK2),nuclear transcription factor κB inhibitor α(IκBα),NF-κB and GDF9 were detected by real-time quantitative polymerase chain reaction(qRT-PCR),and GDF9 protein expression level was detected by Western Blot,and ovarian granulosa cell apoptosis was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling(TUNEL).Results The drug-containing serum of Jinghou Zengzhi Prescription decreased the mRNA expressions of p38MAPK and NF-κB,elevated the mRNA expressions of CK2 and IκBα,increased the mRNA and protein expression levels of GDF9,and decreased the apoptosis rate of ovarian granulosa cells in COH rats.The addition of p38MAPK inhibitor SB203580 alone and the addition of NF-κB inhibitor PDTC alone both promoted the mRNA and protein expressions of GDF9 and reduced the apoptosis rate of granulosa cells.Conclusion The drug-containing serum of Jinghou Zengzhi Prescription based on qi and blood replenishing method can promote the expression of GDF9 and inhibit the apoptosis of ovarian granulosa cells in rats with COH,and its mechanism may be related to the regulation of the expression of genes of the dual signaling pathways of p38MAPK and NF-κB.
