Effects of Astragaloside Ⅳ on High Glucose-Induced Pyroptosis and Invasive Migration of Human Chorionic Trophoblast Cells(HTR-8/SVneo)
10.13359/j.cnki.gzxbtcm.2024.01.027
- VernacularTitle:黄芪甲苷对高糖诱导人绒毛膜滋养层细胞(HTR-8/SVneo)焦亡及侵袭迁移的影响
- Author:
Wen-Hui YE
1
;
Hai-Xia XIAO
;
Shuang-Ming CAI
Author Information
1. 广东省妇幼保健院内科,广东广州 511442
- Keywords:
astragalosideⅣ;
gestational diabetes mellitus;
pyroptosis;
invasion;
migration;
human chorionic trophoblast cells
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(1):178-184
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of astragaloside Ⅳ on high glucose-induced pyroptosis and invasive migration of human chorionic trophoblast cells(HTR-8/SVneo).Methods HTR-8/SVneo cells were divided into 4 groups:control group(untreated),high glucose group(high glucose stimulation)and astragaloside Ⅳ 50 and 100 μmol/L group(high glucose stimulation + astragaloside).Cell activity was detected by Cell Counting Kit 8(CCK-8),cell invasion and migration abilities were determined by Transwell assay and scratch assay,respectively,cell pyroptosis was assessed by Hoechst 33342/propidium iodide(PI)dual fluorescence staining.The protein expression levels of NOD-like receptor thermoprotein structural domain(NLRP3),cleaved-Caspase-1,GSDMD-NT,and IL-18 were detected by Western Blot.Results Compared with the control group,HTR-8/SVneo cell viability was significantly reduced in the high glucose group,the rate of cell migration was significantly reduced,the number of invasive cells was significantly reduced,the percentage of PI-positive cells was significantly increased,and the levels of NLRP3,cleaved-Caspase-1,GSDMD-NT and IL-18 protein expression levels were significantly increased(P<0.05 or P<0.01);compared with the high glucose group,cell viability was significantly higher in the astragaloside Ⅳ treated group,the rate of cell migration was significantly increased,the number of invasive cells was significantly increased,the percentage of PI-positive cells was significantly decreased,and the protein expressions of number of NLRP3,cleaved-Caspase-1,GSDMD-NT,IL-18 were significantly decreased(P<0.05 or P<0.01).Conclusion AstragalosideⅣcan inhibit high glucose-induced HTR-8/SVneo cell pyrolysis and improve cell invasion and migration ability.
