Study on the Effect of Liuwei Dihuang Pills on Regulating the Antigen Cross-Presenting Ability of Dendritic Cells by Interfering with Gap Junctional Communication Function
10.13359/j.cnki.gzxbtcm.2024.01.026
- VernacularTitle:六味地黄丸通过干预缝隙连接通讯功能调控树突状细胞抗原交叉提呈能力研究
- Author:
Yue SONG
1
;
Man-Si XU
;
Xue-Ying ZHONG
;
Wen-Jing ZHANG
;
Xiao-Yi CHEN
;
Biao-Yan DU
;
Jian-Yong XIAO
;
Kun WANG
Author Information
1. 广州中医药大学基础医学院,广东广州 510006
- Keywords:
Liuwei Dihuang Pills;
melanoma;
gap junctional intercellular communication(GJIC);
dendritic cells;
immunocide(DC);
B16 cells
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(1):169-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether Liuwei Dihuang Pills enhances the antigen cross-presenting ability of dendritic cell(DC)by increasing gap junctional intercellular communication(GJIC),and to explore the mechanisms involved.Methods Western Blot and immunofluorescence were used to observe the effects of Liuwei Dihuang Pills-containing serum on the expression and membrane localisation of gap junction protein connexin43(Cx43)in mouse melanoma cells(B16);Calcein-AM/DiI fluorescence tracer assay was used to observe the effects of Liuwei Dihuang Pills-containing serum on the function of GJIC in B16 cells;flow cytometry was used to observe the role of GJIC in the enhancement of DC antigen presenting ability by Liuwei Dihuang Pills-containing serum;and propidium iodide(PI)/Hoechst staining assay was used to observe the immunocidal effect of CD8+ T-lymphocytes.Results Western Blot and immunofluorescence experiments showed that Liuwei Dihuang Pills-containing serum led to the up-regulation of Cx43 expression;fluorescence tracer experiments proved that the GJIC function of B16 cells was significantly enhanced by Liuwei Dihuang Pills-containing serum;flow cytometry analyses showed that the DC antigen-presenting ability was enhanced by Liuwei Dihuang Pills-containing serum;and the results of PI/Hoechst staining showed that the immuno-killing effect of CD8+T-cells was more significant after the intervention of Liuwei Dihuang Pills-containing serum in B16-OVA.Conclusion Liuwei Dihuang Pills improve the GJIC function by up-regulating the Cx43 expression of melanoma cells,and then enhance the cross-presenting ability of DCs thus activating stronger CD8+ T-cell immunocidal responses.
