Optimization of alcohol precipitation process for Quhan Zhufeng Granules by overall desirability and central composite design
10.3760/cma.j.cn115398-20231110-00118
- VernacularTitle:基于总评归一化法结合响应面设计优化祛寒逐风颗粒醇沉工艺
- Author:
Shifu ZHENG
1
;
Zhirui ZHANG
;
Yingyan BI
;
Jiwen LI
;
Xixiang LI
Author Information
1. 甘肃省中医院药学部，兰州 730050
- Keywords:
Technology improving (TCD);
Quhan Zhufeng Granules;
Gentiopicroside;
Oleanolic Acid;
Overall desirability;
Central composite design
- From:
International Journal of Traditional Chinese Medicine
2024;46(4):492-497
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the alcohol precipitation process of Quhan Zhufeng Granules.Methods:Taking the volume fraction of ethanol, the relative density of the concentrated solution and the standing time as the investigation factors, the Z value of the total evaluation of gentiopicrin content, oleanolic acid content and dry paste yield of gentiana macrophylla as the evaluation indexes, the star-point design-response surface method was used to optimize the alcohol precipitation process.Results:The optimal the alcohol precipitation process of Quhan Zhufeng Granules: concentration relative density 1.08 g/ml (90-95 ℃), alcohol precipitation at the end of volume fraction of 62% ethanol, standing for 16 h.Conclusion:The alcohol precipitation process using overall desirability and central composite design is stable and feasible, and has good predictability, which can provide experimental basis for further scale production.
