Effects of Jianpi Bushen Jiedu Prescription regulating JAK2/STAT3 pathway on proliferation and migration of hepatocellular carcinoma cells
10.3760/cma.j.cn115398-20221226-00344
- VernacularTitle:健脾补肾解毒方调控JAK2/STAT3通路对肝癌细胞增殖与迁移的影响
- Author:
Huidi LI
1
;
Yuanyuan FENG
;
Youying LAI
;
Ru JIA
;
Pingping ZHANG
;
Xiaojun ZHU
;
Hongjie LIU
Author Information
1. 上海中医药大学附属曙光医院肿瘤科，上海 201203
- Keywords:
Liver neoplasms;
Spleen-tonifying kidney detoxification prescription;
JAK2/STAT3 pathway;
Cell proliferation;
Cell migration
- From:
International Journal of Traditional Chinese Medicine
2024;46(2):186-190
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of Jianpi Bushen Jiedu Prescription on the proliferation and migration of hepatocellular carcinoma cells; To discuss its possible mechanism.Methods:Using human highly metastatic liver cancer cell line (HCCLM3) as the research object, they were randomly divided into control group and TCM group (100, 200, 400, 800, 1 600, 3 200 μg/ml Jianpi Bushen Jiedu Prescription) and Western medicine group (2.5, 5, 10, 20, 40 μmol/L sorafenib) using a random number table method. Cell viability was detected using cell counting reagent (CCK-8) method; HCCLM3 cells were divided into control group and TCM (Jianpi Bushen Jiedu Prescription 800 μg/ml) group and combined group (Jianpi Bushen Jiedu Prescription 800 μg/ml +sorafenib 20 μmol/L). Western blot method was used to detect the protein expressions of kinase/signaling transducer and transcriptional activator (JAK2/STAT3) pathway related proteins (p-JAK2, JAK2, p-STAT3, STAT3) in each group.Results:Compared with the control group, viability and mobility of HCCLM cell in TCM group and Western medicine group decreased ( P<0.01 or P<0.05); compared with the control group, the protein expressions of P-JAK2, JAK2, P-STAT3 and STAT3 in the TCM group and the combined group decreased ( P<0.05), and the JAK2 protein expression in the combined group was lower than that in the TCM group ( P<0.05). Conclusion:Jianpi Bushen Jiedu Prescription can inhibit the proliferation and migration of HCC cells by regulating JAK2/STAT3 pathway.
