Study on quality evaluation of Buddleja officinalis from different habitats based on HPLC fingerprint and content determination
10.3760/cma.j.cn115398-20230117-00165
- VernacularTitle:基于UPLC指纹图谱和含量测定的不同产地密蒙花药材质量评价研究
- Author:
Cuijie WEI
1
;
Xiaoxia LIU
;
Zhiwen DUAN
;
Yongwei FENG
;
Xiaozhou JIA
;
Yueyi LIANG
;
Xiangdong CHEN
;
Dongmei SUN
;
Zhenyu LI
Author Information
1. 广东一方制药有限公司 广东省中药配方颗粒企业重点实验室，佛山 528244
- Keywords:
Buddlejae Flos;
UPLC fingerprint;
Assay (TCD);
Quality control
- From:
International Journal of Traditional Chinese Medicine
2024;46(2):215-221
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish UPLC fingerprint method and 2 contents determination methods of Buddleja officinalis; To provide a reference for improving the quality control standard and evaluation of Buddleja officinalis from different habitats.Methods:UPLC method was used to establish the fingerprints of 17 batches of Buddleja officinalis. The similarity evaluation, clustering analysis, principal component analysis and orthogonal partial least squares discriminant analysis were used to compare the quality differences of Buddleja officinalis from different habitats. The contents of acteoside and linarin in Buddleja officinalis were determined.Results:There were 12 common peaks in UPLC fingerprints of Buddleja officinalis, six of which were identified as echinacoside, acteoside, cynaroside, isoacteoside, linarin, and apigenin. The fingerprint similarity of 17 batches of Buddleja officinalis was more than 0.9; Buddleja officinalis from different habitats were classified into 2 groups. Five differential markers were determined by OPLS-DA analysis. The order of significance was acteoside > peak 3 > echinacoside > isoacteoside > linarin. Edgeworthia chrysantha was identified by the method of fingerprint as counterfeit. The results of content determination showed that the content of Buddleja officinalis in Hubei and Sichuan was the high and stable.Conclusion:The method can effectively analyze the differences of Buddleja officinalis from different habitats, and provide reference for the quality control of Buddleja officinalis.
