Study on consistency of traditional decoction and formula granules of Sangju Decoction based on fingerprint printing and chemical pattern recognition
10.3760/cma.j.cn115398-20230426-00372
- VernacularTitle:基于指纹图谱结合化学模式识别的桑菊饮传统汤剂与配方颗粒一致性评价研究
- Author:
Yuechen FAN
1
;
Ting CHEN
;
Meizhen LI
;
Wei DAI
;
Yuyu HUANG
Author Information
1. 安徽中医药大学药学院，合肥 230012
- Keywords:
Sang Ju Yin;
Decoctions (TCD);
Formula granule;
Fingerprint;
Cluster analysis;
Principal component analysis;
Orthogonal partial least squares discriminant a
- From:
International Journal of Traditional Chinese Medicine
2024;46(1):69-75
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the chemical composition of decoction and granules of Sangju Decoction; To provide a method for quality evaluation of Sangju Decoction.Methods:HPLC was used to establish fingerprints, and a comprehensive comparative study was conducted on the traditional decoction and formula granules of Sangju Decoction from four aspects: chemical composition type, fingerprint similarity, chemical pattern recognition analysis, and representative index component content.Results:The fingerprint similarity of the 10 batches of traditional decoction was >0.988. 35 peaks were identified and 12 peaks were identified as common peaks (neochlorogenic acid for peak 7, chlorogenic acid for peak 10, cryptochlorogenic acid for peak 11, 1,3-dicaffeoylquinic acid for peak 13, rutin for peak 17, lenoside A for peak 19, lignan for peak 20, isochlorogenic acid B for peak 24, ammonium glycyrrhizate for peak 25). The fingerprint similarity of the formulation pellets was >0.983, and 29 characteristic peaks were identified. Compared with the traditional decoction, some batches of the granules lacked peaks 14, 26, 27, 30, 32 and 34, and clustering analysis (CA), principal component analysis (PCA), and orthogonal partial least squares discriminant analysis (OPLS-DA) could distinguish between the two. The contents of the 10 index components neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, 1,3-dicaffeoylquinic acid, forsythia ester glycoside A, grass glycosides, isochlorogenic acid B, 3,5-O-dicaffeoylquinic acid, forsythia glycosides, monkshood glycosides in the traditional soup were higher than that in the granules, and the contents of rutin and ammonium glycyrrhizate in the granules were higher than that in traditional decoction.Conclusions:The content and composition of traditional decoction and formula granules of Sangju Decoction are significantly different. The combination of fingerprinting and chemical pattern identification effectively can effectively evaluate the difference between traditional decoction and formula granules of Sangju Decoction, which can lay a foundation for the quality control and rational clinical application of formula granules of Sangju Decoction.
