Study on function of Zhibao Sanbian Pills on improving learning and memory impairment in mice based on bahaviour experiment and network pharmacology
10.3760/cma.j.cn115398-20220817-00194
- VernacularTitle:基于行为学实验和网络药理学的至宝三鞭丸改善小鼠学习记忆能力作用研究
- Author:
Guanlian WANG
1
;
Yongmei ZHANG
;
Ran XIE
;
Jialin LIU
;
Lei BAO
;
Chuanwen LIU
;
Pengqian WANG
;
Maobo DU
Author Information
1. 中国中医科学院中药研究所中药制剂中心，北京 100700
- Keywords:
Zhibao Sanbian Pill;
Cognitive impairment;
Behavioral experiment;
Network pharmacology;
Mice
- From:
International Journal of Traditional Chinese Medicine
2023;45(12):1523-1531
- CountryChina
- Language:Chinese
-
Abstract:
Objective:This study aims to investigate the effects of Zhibao Sanbian Pills on learning and memory ability and explore its mechanism.Methods:Cognitive decline and memory impairment in mice was induced by scopolamine. Morris water maze test, platform jumping test and dark avoidance test were used to evaluate the learning and memory ability of mice. TCMSP, TCMID database and literature search were used to search the chemical components of Zhibao Sanbian Pills. The potential active components were screened through ADMETlab 2.0. The targets were predicted by Swiss Target Prediction. GO function and KEGG pathway enrichment analysis were conducted through the Metascape database, and a PPI network was constructed for MCODE module analysis.Results:Compared with the model group, the latency of water maze test in the high-dose group of Zhibao Sanbian Pills was shortened ( P<0.05), and the time of platform quadrant and the number of crossing increased ( P<0.05). 863 active components and 401 potential targets of Zhibao Sanbian Pills were collected. The enrichment analysis of MCODE, GO and KEGG pathways found that the action pathways of Zhibao Sanbian Pills to improve cognitive impairment involve neuroactive ligand receptor pathway, calcium signal pathway, Rap1 signal pathway, PI3K-Akt signal pathway, MAPK signal transduction pathway, 5-hydroxytryptamine receptor pathway, etc. Conclusion:Zhibao Sanbian Pills have the potential to improve the learning and memory ability of mice, which may be realized through MAPK signaling pathway and other neural related signaling pathways.
