Establishment of UPLC fingerprint and quantitative analysis of two components of Buddlejae Flos standard decoction
10.3760/cma.j.cn115398-20221008-00008
- VernacularTitle:密蒙花标准汤剂UPLC指纹图谱建立及2种成分含量测定分析研究
- Author:
Cuijie WEI
1
;
Xiaoxia LIU
;
Zhiwen DUAN
;
Yongwei FENG
;
Fangping ZHANG
;
Chunxiu WU
;
Xiangdong CHEN
;
Dongmei SUN
;
Zhenyu LI
Author Information
1. 广东一方制药有限公司 广东省中药配方颗粒企业重点实验室，佛山 528244
- Keywords:
Buddlejae Flos;
Standard decoction;
Fingerprint;
Assay (TCD);
Edgeworthia chrysantha
- From:
International Journal of Traditional Chinese Medicine
2023;45(12):1542-1548
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish UPLC fingerprint method of Buddlejae Flos standard decoction and determination method of acteoside and linarin.Methods:UPLC method was used to establish the fingerprints of 17 batches of Buddlejae Flos standard decoction. Similarity evaluation and clustering analysis were carried out on the fingerprints of Buddlejae Flos standard decoction; the chromatographic peaks of standard decoction were identified by mass spectrometry and compared with the reference materials; the contents of acteoside and linarin in Buddlejae Flos standard decoction were determined by HPLC.Results:There were 11 common peaks in the fingerprint of Buddlejae Flos standard decoction and 6 of them were identified. The similarity of the 17 batch samples was between 0.972 and 0.999. Clustering analysis classified 17 batches of Buddlejae Flos standard decoction into two categories; edgeworthia chrysantha standard decoction was identified by the method of fingerprint as counterfeit; the content determination results showed that the contents of acteoside and linarin in the standard decoction prepared from Buddlejae Flos of in Hubei and Sichuan Provinces were higher than others and were more stable.Conclusion:The method can be used to comprehensively evaluate the quality of Buddlejae Flos standard decoction and provide reference for establishing the quality standard of Buddlejae Flos dispensing granules.
