Study on effects of Jianpi Qinghua Decoction on hepatocyte apoptosis in nonalcoholic fatty liver disease model mice
10.3760/cma.j.cn115398-20221129-00498
- VernacularTitle:健脾清化方对非酒精性脂肪性肝病模型小鼠细胞凋亡的影响
- Author:
Xu HAN
1
;
Mengjie CAI
;
Qingguang CHEN
;
Jing TIAN
;
Hao LU
Author Information
1. 上海中医药大学附属曙光医院内分泌科，上海 201203
- Keywords:
Non-alcoholic fatty liver disease;
Jianpi Qinghua Decoction;
Hepatocytes;
Apoptosis;
Mice
- From:
International Journal of Traditional Chinese Medicine
2023;45(11):1382-1385
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of Jianpi Qinghua Decoction on hepatocyte apoptosis under non-alcoholic fatty liver disease (NAFLD), and to discuss its mechanism.Methods:Totally 29 C57 mice were randomly selected and fed a 60% high fat diet for 16 weeks, while the remaining 6 mice were given regular feed as the normal group. After successful modeling, 12 mice with larger body weight were divided into TCM group and model group using a random number table method, while continuing to receive high-fat feed. The TCM group was orally administered Jianpi Qinghua Decoction 20.961 g/kg. The normal group and model group were orally administered an equal volume of distilled water once a day, with continuous intervention for 4 weeks. The levels of GOT and GPT in blood were detected by ELISA, the deposition of triglyceride in liver was detected by oil red O, and the apoptosis of liver cells was detected by TUNEL fluorescence staining. Western blot was used to detect the expressions of cleaved Caspase-3, Caspase-3, Bax, Bcl-2, JNK and p-JNK.Results:Compared with the model group, the body weight and GPT in the TCM group significantly decreased ( P<0.05), TG deposition was significantly reduced, apoptosis range of liver cells was significantly reduced, and cleaved Caspase-3/Caspase-3, p-JNK/JNK and the expression of Bax significantly decreased ( P<0.05). The expression of Bcl-2 increased ( P<0.05). Conclusion:Jianpi Qinghua Decoction can inhibit JNK protein phosphorylation and effectively reduce liver cell apoptosis in NAFLD mice, which may delay the progression of NAFLD towards cirrhosis and liver cancer.
