Contrastive analysis on difference of chemical compositions of single decoction and mixed decoction of Gypsum Fibrosum-Anemarrhenae Rhizoma medicinal pair with different proportion
10.3760/cma.j.cn115398-20220917-00230
- VernacularTitle:不同配伍比例石膏-知母药对合煎与单煎化学成分差异性研究
- Author:
Yingxin ZHOU
1
;
Yao HUANG
;
Xiaoxuan YAO
;
Tian TIAN
;
Jiaming LIN
;
Jianyan SUN
;
Zhipeng ZHANG
;
Dongmei SUN
Author Information
1. 广东一方制药有限公司 广东省中药配方颗粒企业重点实验室，佛山 528244
- Keywords:
Gypsum Fibrosum;
Anemarrhenae Rhizoma;
Couplet medicines;
Compatibility (TCD);
Decoction preparing methods;
Fingerprint
- From:
International Journal of Traditional Chinese Medicine
2023;45(10):1281-1287
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the difference of chemical compositions of single decoction and mixed decoction of Gypsum Fibrosum - Anemarrhenae Rhizoma medicinal pair with different proportion.Methods:The Ultra Performance Liquid Chromatography (UPLC) and Ion Chromatography (IC) fingerprints of each sample were collected, and the independent sample t-test on the ratio of "peak area/weight" value of common peaks which was from the single decoction and mixed decoction samples was performed through SPSS 26.0.Results:In the UPLC fingerprint, there was significant difference in the "peak area/weight" values of peak 1, peak 2, peak 3, peak 6 and peak 13 between mixed decoction and single decoction with different compatibility ratios (1:1, 3:2, 2:1, 5:2, 3:1)( P<0.05), while peak 5 and peak 11 were without significant difference ( P>0.05). When the compatibility ratio of Gypsum Fibrosum and Anemarrhenae Rhizoma was 1:1, 3:2, 5:2, 3:1, there was significant difference in the "peak area/weight" values of peak 7 (neomangiferin) ( P<0.05). In the IC fingerprint, there was significant difference in the "peak area/weight" values of peak 1 between mixed decoction and single decoction with different compatibility ratios (1:1, 3:2, 2:1, 5:2, 3:1) ( P<0.05), while there was no significant difference in peak 5 (calcium ion) ( P>0.05). Conclusion:There are differences in the chemical component content of Gypsum Fibrosum-Anemarrhenae Rhizoma medicinal pair for single decoction and mixed decoction.
