Expression of PLCD3 mRNA in synovium of osteoarthritis and its relationship with immune cell infiltration
10.3969/j.issn.1673-4130.2024.02.017
- VernacularTitle:骨关节炎滑膜中PLCD3 mRNA的表达情况及其与免疫细胞浸润的关系
- Author:
Pu YING
1
;
Zhi ZHENG
;
Yue XU
;
Ye ZHOU
;
Yufan GE
;
Yi XUE
;
Yiming MIAO
Author Information
1. 南京中医药大学常熟附属医院骨科,江苏常熟 215500
- Keywords:
osteoarthritis;
phospholipase Cδ3;
immune cell infiltration
- From:
International Journal of Laboratory Medicine
2024;45(2):208-212
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of PLCD3 mRNA in the synovium of osteoarthritis(OA)and its relationship with immune cell infiltration.Methods Based on the differentially expressed genes of OA found in the previous study,the expression of phospholipase Cδ3(PLCD3)mRNA was detected by col-lecting synovial samples from OA group and control group.CIBERSORT algorithm was used to analyze the infiltration pattern of immune cells in OA group and control group,and the correlation between PLCD3 and infiltrating immune cells was further analyzed.Results Compared with the control group,the relative expres-sion level of PLCD3 mRNA was significantly increased in synovial samples of OA group(P<0.05).The pro-portions of B cells naive,NK cells activated,M2 macrophages and mast cells activated in synovial tissues of OA group were relatively high(P<0.05).PLCD3 was positively correlated with the proportion of these four immune cells(P<0.05).Conclusion PLCD3 may be a key biomarker for the diagnosis of OA,which may be involved in the pathogenesis of OA by interacting with infiltrating immune cells.
