Analysis of the relationship between Salmonella drug sensitivity and ion peak by mass spectrometry
10.3969/j.issn.1673-4130.2023.24.016
- VernacularTitle:沙门菌药敏结果与质谱离子峰的关系分析
- Author:
Fengyuan HE
1
;
Bo TAN
;
Ming LI
;
Qiwu YUAN
;
Limin MEI
;
Lin ZHAO
Author Information
1. 成都市郫都区疾病预防控制中心,四川成都 611730
- Keywords:
Salmonella;
matrix assisted laser desorption ionization time-of-flight mass spectrometry;
drug resistance
- From:
International Journal of Laboratory Medicine
2023;44(24):3027-3031,3036
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between drug susceptibility of Salmonella bacteria and ion peak by mass spectrometry.Methods A total of 19 strains of Salmonella collected from the laboratory of Chengdu Center for Disease Control and Prevention from 2017 to 2020 were selected as the research objects.Serotyping and drug sensitivity tests were performed.The protein fingerprints of Salmonella were detected by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.The relationship between drug sensitivity test and mass spectrometry in serotyping and drug resistance type identification was compared.Re-sults A total of 19 strains of Salmonella were generally resistant to β-lactam,and the antimicrobial resistance rate was above 50.00%except for cefepime,cefotetan and ertapenem.It was generally sensitive to non-β-lac-tam antibiotics,and the sensitivity rate was higher.The results of matrix assisted laser desorption ionization time-of-flight mass spectrometry showed that 3 128,3 158,5 144,6 094,64 84 m/z might be the specific pro-tein peaks of imipenem resistant Salmonella,5 772 m/z might be the specific protein peak of tobramycin re-sistant salmonella,3 046 m/z might be the specific protein peak of Salmonella resistant to levofloxacin,4 165 m/z might be the characteristic peak of Salmonella resistant to cefepime,10 957 m/z might be the common characteristic peak of Salmonella resistant to imipenem,cefazolin and ceftazidime,5 710 m/z might be the common characteristic peak of Salmonella resistant to tobramycin and cefotetan,4 165 m/z may be the com-mon characterstic peak of Salmonella resistant to imipenem and levofloxacin.Conclusion This study prelimi-narily explored the drug resistance spectrum of salmonella fingerprint results,which can provide reference for clinical drug use in time.
