P16/Ki-67 double dye the value of diagnosis of cervical intraepithelial lesions and cervical biopsy consistency analysis
10.3969/j.issn.1673-4130.2023.24.005
- VernacularTitle:P16/Ki-67双染诊断宫颈上皮内病变的价值及与宫颈活检的一致性分析
- Author:
Xiuhong QIAN
1
;
Chengmin QIU
Author Information
1. 上海市松江区中心医院病理科,上海 201600
- Keywords:
cervical intraepithelial lesion;
P16/Ki-67 double dye;
high-risk human papillomavirus;
cervical biopsy
- From:
International Journal of Laboratory Medicine
2023;44(24):2970-2974
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of P16/Ki-67 double dye in the diagnosis of cervical intraepithe-lial lesion(CIN)value,and to analyze its consistency with cervical biopsy.Methods A total of 92 patients with suspected CIN in Shanghai Songjiang District Central Hospital from November 2021 to April 2023 were selected as the research objects.All patients underwent high-risk human papillomavirus(HR-HPV)and P16/Ki-67 double dye.Taking cervical biopsy as the gold standard,the diagnostic value of the two detection meth-ods for CIN was analyzed,as well as the consistency of the two detection methods in the diagnosis of CIN clas-sification and cervical biopsy.Results The results of cervical biopsy showed that 74 cases(80.43%)of 92 suspected CIN patients were positive,including 26 cases of CIN Ⅰ,32 cases of CIN Ⅱ and 16 cases of CIN Ⅲ,and 18 cases(19.57%)were negative.Taking cervical biopsy results as the gold standard,P16/Ki-67 double dye was more accurate than HR-HPV test in the diagnosis of CIN(P<0.05).The receiver operating charac-teristic(ROC)curve showed that the area under the curve of HR-HPV and P16/Ki-67 double dye for CIN di-agnosis was 0.760 and 0.890,respectively,all of them had certain diagnostic value.The consistency between HR-HPV evaluation of CIN classification and cervical biopsy was consistent(Kappa=0.707,P<0.05).The consistency between P16/Ki-67 double dye and cervical biopsy was excellent(Kappa=0.832,P<0.05).Con-clusion P16/Ki-67 double dye with a high diagnostic value in CIN,consistency in determining CIN grade and cervical biopsy is wonderful,which can provide reference for clinical diagnosis.
