Detection of Amantadine by Label-free Fluorescence Method Based on Truncated Aptamer and Molybdenum Disulfide Nanosheet Signal Enhancement Strategy
10.19756/j.issn.0253-3820.231127
- VernacularTitle:基于截短适配体-二硫化钼纳米片信号增强免标记荧光检测金刚烷胺
- Author:
Yi-Feng LAN
1
;
Bo-Ya HOU
;
Zhi-Wen WEI
;
Wen LIU
;
Chao ZHANG
;
Ya-Hui ZUO
;
Ke-Ming YUN
Author Information
1. 山西医科大学法医学院,晋中 030600
- Keywords:
Truncated aptamer;
Molybdenum disulfide nanosheet;
Signal enhancement;
Amantadine;
Label-free fluorescence sensing system
- From:
Chinese Journal of Analytical Chemistry
2024;52(2):208-219,中插4-中插7
- CountryChina
- Language:Chinese
-
Abstract:
Amantadine(AMD)residue can accumulate in organisms through the food chain and cause serious harm to human body.AMD can specifically bind to AMD specific aptamer and cause its conformation to change from a random single strand to a stem-loop structure.To avoid the influence of excess nucleotides on binding of aptamer to AMD,the truncation of the AMD original aptamer J was optimized by retaining an appropriate stem-loop structure,and a new type of truncation aptamers was developed in this work.By comparing the truncated aptamer with the original aptamer,it was found that the truncated aptamer J-7 had better affinity and specificity with AMD.The detection limit of AMD was 0.11 ng/mL by using J-7 as specific recognition element and molybdenum disulfide nanosheet(MoS2Ns)as signal amplification element.The developed method base on truncated aptamer J-7 was used for detection of AMD in milk,yogurt and SD rat serum samples for the first time with recoveries of 86.6%-108.2%.This study provided a reference for truncating other long sequence aptamers and provided a more sensitive detection method for monitoring AMD residues in food.
