Rapid Determination of 32 Kinds of Veterinary Drug Residues in Eggs Using Modified QuEChERS Based on Reduced Graphene Oxide-coated Melamine Sponge by Ultra-High Liquid Chromatography-Tandem Mass Spectrometry
10.19756/j.issn.0253-3820.231318
- VernacularTitle:基于还原氧化石墨烯改性三聚氰胺海绵的改良QuEChERS-超高效液相色谱-串联质谱法快速测定鸡蛋中32种兽药残留
- Author:
Xu XU
1
,
2
,
3
;
Jia LYU
;
Lan-Rui YANG
;
Zhu-Chen HOU
;
Bao-Cheng JI
;
Yan-Hong BAI
Author Information
1. 郑州轻工业大学食品与生物工程学院,郑州 450001
2. 郑州轻工业大学河南省冷链食品安全质量安全控制重点实验室,郑州 450001
3. 郑州轻工业大学冷链食品加工与安全控制教育部重点实验室,郑州 450001
- Keywords:
Veterinary drug;
Egg;
Graphene oxide;
Melamine sponge;
Liquid chromatography-tandem mass spectrometry
- From:
Chinese Journal of Analytical Chemistry
2024;52(1):121-129,中插38-中插43
- CountryChina
- Language:Chinese
-
Abstract:
A rapid analytical method for simultaneous determination of 32 kinds of multi-residue veterinary drugs in eggs was developed using a modified QuEChERS technique based on a reduced graphene oxide-coated melamine sponge(r-GO@MeS)by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The influences of graphene oxide(GO)concentrations,sponge dosages,and purification modes on drug recoveries were investigated during the purification process.The optimal purification conditions involved using a GO concentration of 0.5 mg/mL,a sponge dosage of 6.0 cm3/mL,and a dynamic purification mode of 5 extrusion cycles.Separation was achieved using an Agilent Eclipse Plus C18 RRHD column(100 mm×2.1 mm,1.8 μm),and quantitative analysis was performed by the external standard method using an electrospray ionization source(ESI)in multiple reaction monitoring(MRM)mode.The results showed that all 32 kinds of veterinary drugs exhibited good linear correlation with coefficients greater than 0.999,and matrix effects(MEs)ranging from?7.8%to 18.9%.The limits of detection(LODs)and quantification(LOQs)ranged from 0.2 to 10.2 μg/kg and from 0.6 to 28.0 μg/kg,respectively.The recoveries for the three spiked levels were in the range of 66.5%?117.5%,with intra-day and inter-day precision(Relative standard deviation)below 13.3%and 16.3%,respectively.The synthetic r-GO@MeS exhibited efficient matrix purification without the need of high-speed centrifugation or strong magnetic field assistance.This significantly shorted the sample pretreatment time and improved the convenience of the matrix purification process.Combined with UPLC-MS/MS,the method was suitable for the rapid determination of multi-residue veterinary drugs in eggs.
