In Situ Labeling of Erythrocyte Membrane Anion Channel Proteins with Gold Nanoparticles Observed by Cryo-Scanning Electron Microscopy
10.19756/j.issn.0253-3820.231453
- VernacularTitle:利用冷冻扫描电子显微镜观察金纳米颗粒原位标记红细胞膜阴离子通道蛋白
- Author:
Si-Hang CHENG
1
,
2
;
Hui-Li WANG
;
Yang YU
;
Jin-Rui ZHANG
;
Hong-Da WANG
Author Information
1. 中国科学院长春应用化学研究所,电分析化学国家重点实验室,长春 130022
2. 中国科学技术大学应用化学与工程学院,合肥 230026
- Keywords:
Band 3 protein;
Cryo-scanning electron microscopy;
Gold nanoparticles;
In situ labeling;
Protein distribution;
Protein islands
- From:
Chinese Journal of Analytical Chemistry
2024;52(1):54-61
- CountryChina
- Language:Chinese
-
Abstract:
Band 3 protein is an important channel protein in the erythrocyte membrane which mediates the anion transport process inside and outside the cell membrane,as well as contributes to the maintenance of erythrocyte morphology,and has important physiological functions.However,the distribution state of this protein in the primary cell membrane is not known.Cryo-scanning electron microscopy enables imaging of the surface morphology of biological samples in a near-physiological state.In order to investigate the distribution of band 3 protein on erythrocyte membranes under physiological conditions,the present study utilized 5-nm gold nanoparticles modified with the antibodies to specifically bind to the band 3 protein on human blood erythrocyte membranes and imaged them by cryo-scanning electron microscopy,to obtain distribution of band 3 protein on human blood erythrocyte membranes.The results showed that the membrane proteins on the erythrocyte membranes tended to be clustered and distributed to form ″protein islands″,and band 3 proteins were mainly distributed in these protein islands,which were tightly connected with each other to form several functional microregions to play their respective roles.
