The expression changes of TLR4/MyD88/NF-κB signaling pathway in a mouse model of olfactory dysfunction
10.16066/j.1672-7002.2024.02.010
- VernacularTitle:TLR4/MyD88/NF-κB信号通路在嗅觉障碍小鼠模型中的表达变化
- Author:
Qiong FAN
1
;
Xiaoning ZHU
;
Hongzhou GE
;
Zhijun WANG
Author Information
1. 青岛市第五人民医院耳鼻咽喉科,山东 青岛 266002
- Keywords:
Olfactory Pathways;
Toll-Like Receptor 4;
Myeloid Differentiation Factor 88;
NF-kappa B;
Olfaction Disorders;
Models,Animal
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2024;31(2):113-116
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the expression changes of TLR4/MyD88/NF-κB signaling pathways in olfactory disorders.METHODS There were 40 healthy BALB/c mice who were divided into an observation group and a control group,with 20 mice in each group.Detection of Toll-like receptors(TLR4),myeloid differentiation primary response gene 88(MyD88)and nuclear factor kappa B(NF-κB)in mice using quantitative reverse transcription PCR level;Detection of TLR4,MyD88 and NF-κB by Western blot(WB)test protein content;Immunohistochemical detection of the expression of mouse olfactory marker protein(OMP).RESULTS There was no significant difference in foraging time between the two groups of mice before modeling(P>0.05),after modeling,the foraging time of the observation group mice was significantly longer than that of the control group(P<0.05);The relative mRNA expression level of TLR4,MyD88 and NF-κB in the nasal epithelium of mice in the observation group was significantly higher than that of the control group(P<0.05);The protein expression of TLR4,MyD88 and NF-κB in the nasal epithelium of mice in the observation group was significantly higher than that of the control group(P<0.05);The level of OMP protein in the nasal epithelium of the observation group was significantly lower than that of the control group(P<0.05).CONCLUSION Expression reinforcement of TLR4/MyD88/NF-κB signaling pathway in a mouse model of olfactory dysfunction.
