Effect of CIP2A knockdown on proliferation and migration of hypopharyngeal cancer cells
10.16066/j.1672-7002.2024.01.003
- VernacularTitle:敲低蛋白磷酸酶2A的癌性抑制因子表达对下咽癌细胞增殖、迁移的影响
- Author:
Yao CHENG
1
;
Xi ZHANG
;
Qicheng DENG
;
Hai LIU
Author Information
1. 川北医学院附属医院耳鼻咽喉头颈外科,四川 南充 637000
- Keywords:
Hypopharyngeal Neoplasms;
Cell Proliferation;
Cell Migration Assays
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2024;31(1):13-17
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE The expression of cancerous inhibitor of protein phosphatase 2A(CIP2A)in hypopharyngeal carcinoma FaDu cells(FaDu cells)was reduced by shRNA to understand its role in the occurrence and development of hypopharyngeal carcinoma.METHODS Specific shRNA sequence was designed,lentivirus was packaged and transfected into hypopharyngeal carcinoma FaDu cells,and CIP2A expression was specifically knocked down.The expression of CIP2A was detected by RT-PCR and Western blot.RESULTS 1.After shRNA knocked down CIP2A in FaDu cells,the CIP2A mRNA expression in the experimental group(CIP2A knocked down group)was significantly lower than that in the blank group,and the CIP2A protein expression in the experimental group was also significantly lower than that in the blank group.2.Cell cloning and CCK8 experiments showed that the cell proliferation ability of the experimental group was significantly decreased compared with that of the blank group(t=50.86,P<0.01;t=12.406,P<0.001);The results of cell scratch test showed that the transverse migration ability of the experimental group was significantly decreased compared with the blank group,and the longitudinal migration ability of the experimental group was significantly decreased compared with the blank group by Transwell test(t=40.038,P<0.01;t=12.247,P<0.001).CONCLUSION After knockdown CIP2A expression in hypopharyngeal carcinoma FaDu cells,the proliferation and migration ability of hypopharyngeal cancer cells decreased,suggesting that CIP2A is involved in regulating the biological behavior of hypopharyngeal cancer cells and can be used as a potential anticancer target.
