The effect of growth hormone on invasion and migration of colon cancer cells
10.3969/j.issn.1671-8348.2024.05.005
- VernacularTitle:生长激素对结肠癌细胞侵袭和迁移的影响
- Author:
Haotian JIANG
1
,
2
,
3
,
4
;
Pan WANG
;
Bin LIAO
;
Sheng GONG
;
Nan WU
Author Information
1. 重庆医科大学,重庆 400016
2. 中国科学院重庆绿色智能技术研究院,重庆 400714
3. 中国科学院大学重庆学院,重庆 400714
4. 重庆市人民医院神经外科,重庆 401147
- Keywords:
growth hormone-type pituitary adenomas;
growth hormone;
colon cancer;
invasion;
migration
- From:
Chongqing Medicine
2024;53(5):664-668,676
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of growth hormone(GH)on the proliferation,cycle,inva-sion,and migration of colon cancer cells and its possible mechanism.Methods GH3 cells with growth hor-mone-type pituitary adenoma were cultured in vitro,and the secretion of growth hormone in the supernatant of GH3 cells was detected by ELISA.Colon cancer LoVo cells in logarithmic growth phase were randomly divid-ed into the control group and the experimental group.PBS was added to the control group,while high concen-trations of recombinant GH were added to the experimental group.The two groups of cells were cultured in vitro under the same conditions.CCK-8 method was used to detect the proliferation of the cells.Flow cytome-try was used to detect the cell cycle.Transwell assay was used to detect the effect of growth hormone on the invasion and migration of the cells.Western blot was used to detect the expressions levels of E-cadherin,N-cadherin,Vimentin,and Snail-1 proteins in the cells.Results The results of ELISA showed that GH3 cells could secrete a large amount of GH,and the concentration of GH in the supernatant was(1 208±9)ng/mL.GH promoted cell growth in a dose-dependent manner within a certain concentration range,and GH 200 ng/mL was the optimal intervention concentration for subsequent experiments.Compared with the control group,the cell cycle in the experimental group changed from G1 phase to S phase and G2 phase,the ratio of G1 phase cells decreased,and the ratio of S phase cells and G2 phase cells increased(P<0.05).Compared with the control group,the number of the cell invasion and migration increased in the experimental group(P<0.05),the expression levels of N-cadherin,Vimentin,and Snail-1 was up-regulated,while the expression level of E-cadherin was down-regulated(P<0.05).Conclusion High concentration of GH promotes the prolifera-tion,invasion and migration of colon cancer cells,and induces the transition of cell cycle from G1 to S and G2 phases.The mechanism may be related to the epithelial-mesenchymal transition(EMT)of colon cancer cells promoted by high concentration of GH.
