Preliminary study on expression of LINC02695 in neovascularization in diabetic retinopathy
10.3969/j.issn.1671-8348.2024.04.002
- VernacularTitle:LINC02695表达在糖尿病视网膜病变新生血管中的初步研究
- Author:
Yuan YUAN
1
,
2
;
Anmin ZHU
;
Lan ZENG
;
Xiaofeng LONG
;
Meng YE
;
Kai TANG
;
Wei TAN
Author Information
1. 遵义医科大学第一临床学院,贵州遵义 563000
2. 遵义市第一人民医院/遵义医科大学第三附属医院眼科,贵州遵义 563000
- Keywords:
long non-coding RNA LINC02695;
diabetic retinopathy;
human retinal endothelial cells;
proliferation;
migration;
neovascularization
- From:
Chongqing Medicine
2024;53(4):487-492,497
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of long non-coding RNA(lncRNA)LINC02695 in human retinal microvascular endothelial cells(HRMECs)in high glucose(HG)environment and its effect on the proliferation,migration and neovascularization of HRMECs.Methods HRMECs was divided into four groups:the normal glucose(NG)group(5.5 mmol/L),the HG group(30.0 mmol/L),the HG+LINC02695 silenced group(HG+si-LINC02695),and the HG+silenced control group(HG+si-NC).Real-time quantita-tive fluorescent PCR(qPCR)was used to detect the expression of LINC02695 and vascular endothelial growth factor(VEGF)mRNA in HRMECs of each group.The cell proliferation of each group was measured by Cell Counting Kit-8(CCK-8)method.The migration ability of cells in each group was detected by Transwell as-say.The tube forming ability of cells in each group was detected by tube forming experiment.Results The qPCR results showed that compared with the NG group,LINC02695 and VEGF were highly expressed in the HG group(P＜0.05).Compared with the HG group,VEGF mRNA expression level in the HG+si-LINC02695 group was significantly decreased(P＜0.05).The results of CCK-8 experiment showed that the proliferation ability of the HG group was significantly enhanced compared with the NG group(P＜0.05).Compared with the HG group,the cell proliferation ability of the HG+si-LINC02695 group was significantly decreased(P＜0.05).The results of Transwell experiment showed that the cell migration ability of the HG group was significantly increased compared with the NG group(P＜0.05).Compared with the HG group,the cell migration ability of the HG+si-LINC02695 group was significantly decreased(P＜0.05).The results of tube formation experiment showed that compared with the NG group,the tube formation ability of the HG group was significantly increased(P＜0.05).Compared with the HG group,canalization ability of cells in the HG+si-LINC02695 group was significantly decreased(P＜0.05).Conclusion LINC02695 may be involved in promoting the proliferation,migration and angiogenesis of HRMECs induced by HG.
