Transcriptomic analysis and antagonist screening of cisplatin resistance in ovarian cancer
10.3969/j.issn.1671-8348.2024.04.001
- VernacularTitle:卵巢癌顺铂耐药转录组学分析和拮抗剂筛选的研究
- Author:
Xiaoxiao WANG
1
;
Jiahui DU
;
Lirong LI
;
Weiqiang GUO
;
Songbai LIU
Author Information
1. 苏州卫生职业技术学院苏州检验医学生物技术重点实验室,江苏苏州 215009
- Keywords:
cisplatin;
small molecule inhibitor;
ovarian cancer;
RNA-seq;
drug resistance mechanism
- From:
Chongqing Medicine
2024;53(4):481-486
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the transcriptome differences of ovarian cancer cells after cisplatin(DDP)resistance,and to find potential antagonists based on this screening.Methods DDP-resistant cell line A2780-DDP was constructed with A2780 cells as the research object.Through transcriptome sequencing anal-ysis,the key factors of DDP resistance were found and verified by quantitative real-time PCR(qPCR)and Western blot experiments.Through the screening of small molecule inhibitors,CCK-8 cell viability assay was used to find potential antagonists.Results A2780-DDP were successfully constructed,and it was found that there was no difference in cell proliferation after drug resistance,but the ability of cell invasion and migration was enhanced.Through transcriptome sequencing analysis,it was found that ITGB7 and Akt may be the key genes of A2780-DDP,and qPCR and Western blot showed that they were highly expressed in A2780-DDP.CCK-8 results showed that triptolide(TPL)and Olaparib had good inhibitory effects in DDP-resistant cell lines.Conclusion The ITGB7/Akt pathway plays an important role in DDP resistance,and potential DDP re-sistance antagonists such as TPL can provide new ideas for the treatment of ovarian cancer.
