Heme oxygenase-1 enhances the anti-HBV effect of IFN-αby inducing the expression of antiviral proteins
10.19405/j.cnki.issn1000-1492.2024.02.023
- VernacularTitle:血红素加氧酶-1通过诱导抗病毒蛋白的表达增强IFN-α抗HBV效应
- Author:
Wei DA
1
;
Qin WANG
;
Anbang WEI
;
Hao ZHANG
;
Renbing WANG
;
Qian LIU
;
Qiang ZHOU
Author Information
1. 安徽医科大学第二附属医院检验科,合肥 230601
- Keywords:
hemin;
heme oxygenase-1;
JAK/STAT;
IFN-β
- From:
Acta Universitatis Medicinalis Anhui
2024;59(2):324-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of heme oxygenase-1(HO-1)on HBV replication and the antiviral effect of HO-1 combined with α-interferon(IFN-α).Methods HepG2.2.15 cells and HBV1.3-transfected HepG2 cells(HepG2-HBV1.3)were used as HBV replicating cell models;Hemin treated HepG2.2.15 and HepG2-HBV1.3 cells,to induce the expression of HO-1 molecules.CCK-8 method was used to assess the toxic effects of Hemin on HepG2 and HepG2.2.15;chemiluminescence method was used to analyze HBsAg and HBeAg in the supernatants of Hemin-treated group and si-HO-1 and other experimental groups;RT-qPCR was used to ana-lyze HO-1,IFN-β and HBV-DNA;Western blot was used to analyze the expression of IRF-3 and the expression of related molecules in the JAK/STAT signaling pathway;Hemin combined with IFN-α treated HepG2.2.15 to moni-tor whether HO-1 had synergistic IFN-α antiviral effect.Results Hemin dose-dependently induced HO-1,and HO-1 was induced to exert a significant anti-HBV effect,while the expression of IFN-β,IRF-3,and IRF-9 and MxA,downstream molecules of the JAK/STAT signaling pathway,were all increased.Silencing HO-1 expression reversed the antiviral effect in the Hemin-induced group,and at the same time,type Ⅰ interferon IFN-β showed low expression,and the expression of IRF-9 and MxA in the JAK/STAT signaling pathway was inhibited as well.He-min combined with IFN-α exerted stronger antiviral effects.Conclusion HO-1 can exert an anti-HBV effect,which may be due to increased phosphorylation of IRF-3 to induce type Ⅰ interferon expression and thus activate the JAK/STAT signaling pathway to exert an antiviral effect;HO-1 can synergize with IFN-α to exert an antiviral effect.
