Using Mendelian randomization to explore the causal association between gut microbiota and nonalcoholic fatty liver disease
10.19405/j.cnki.issn1000-1492.2024.02.009
- VernacularTitle:运用孟德尔随机化探索肠道微生物与非酒精性脂肪性肝病的因果关联
- Author:
Min WU
1
;
Zhenxiang AN
;
Yuanli HE
;
Song HE
;
Chuang LIU
;
Kai SUN
Author Information
1. 贵州中医药大学第一临床医学院,贵阳 550001
- Keywords:
gut microbiota;
non-alcoholic fatty liver disease;
Mendelian randomization
- From:
Acta Universitatis Medicinalis Anhui
2024;59(2):236-242
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the causal association between gut microbes and non-alcoholic fatty liver disease(NAFLD)by Mendelian randomisation analysis.Methods Genetic instrumental variables for gut microbiota were identified from a gene-wide association study of 18 340 participants,and summary statistics for NAFLD were ob-tained from the FinnGen database,which provided data on 894 NAFLD cases and 217 898 controls using the IVW method as the primary analysis.In order to test the robustness of the results,MR-Egger method,WM method,Simple Mode method,Weighted Mode method were used for Mendelian randomisation analysis,and heterogeneity test,sensitivity analysis,and multiplicity analysis were performed.Results class Gammaproteobacteria IVW re-sults showed(OR=0.621,95％CI=0.412～0.934,P=0.022);family Enterobacteriaceae IVW results showed(OR=1.481,95％CI=1.069～2.053,P=0.018);genus Lachnospiraceae IVW results showed(OR=1.405,95％CI=1.036～1.904,P=0.029);genus Prevotella7 IVW results showed(OR=0.834,95％CI=0.714～0.974,P=0.021);genus Prevotella9 IVW results showed(OR=1.251,95％CI=1.025～1.527,P=0.027);order Desulfovibrionales IVW results showed(OR=0.714,95％CI=0.519～0.982,P=0.038);or-der Enterobacteriales IVW results showed(OR=1.481,95％CI=1.069～2.053,P=0.018).And there was no heterogeneity in the heterogeneity test,and the sensitivity analyses all showed robustness and no pleiotropy was found.Conclusion This study implicates class Gammaproteobacteria,family Enterobacteriaceae,genus Lachno-spiraceae,genus Prevotella7,genus Prevotella9,order Desulfovibrionales,order Enterobacteriales seven species of gut microorganisms have a causal relationship with NAFLD.
