Morin ameliorates chronic obstructive pulmonary disease by inhibiting the expression of MMP9
10.19405/j.cnki.issn1000-1492.2023.12.001
- VernacularTitle:桑色素通过抑制MMP9表达改善慢性阻塞性肺疾病
- Author:
Anming LENG
1
;
Jing YANG
;
Kui ZHANG
Author Information
1. 贵州省人民医院中医科,贵阳 550002
- Keywords:
chronic obstructive pulmonary disease;
morin;
network pharmacology;
MMP9;
inflammatory reaction
- From:
Acta Universitatis Medicinalis Anhui
2023;58(12):1987-1994
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of morin on chronic obstructive pulmonary disease(COPD)animal and cell model through the regulation of matrix metalloproteinase-9(MMP9).Methods SwissTargetPrediction da-tabase was used to predict the genetargets of morin,GeneCards,OMIMand DisGenet databases were used to predict the risk genes of COPD,the intersection of COPD-related gene targets and morin gene targets was obtained by using Venny online website;Protein-protein interaction(PPI)analysis was performed using the String database,and to-pological analysis was performed by Cytoscape 3.8.2 software.Molecular docking was used to validate the interac-tion between the drug components and gene targets;COPD rat models were constructed to verify the therapeutic effects of morin om COPD rat models.The COPD cell model was established to interfere with the expression of MMP9 in the cells.COPD cell models were established and the expression of MMP9 in cells was intervened;flow cytometry was used to detect cell apoptosis rate;ELISA was used to measure the concentration of inflammatory fac-tors.Results The Swiss Target Prediction database predicted 96 gene targets of morin.GeneCards,OMIM,and DisGenet databases predicted 7 122 gene targets of COPD,intersection analysis identified 67 common gene targets between morin and COPD.Topological analysis revealed that the top 5 gene targets with strongest interactions a-mong the intersected targets were SRC,MMP9,MMP2,PTGS2,and FURIN;Molecular docking results showed the best binding activity between MMP9 and morin;animal experiments showed that morin improved respiratory function parameters and lung tissue pathological damage,and inhibited MMP9 expression in COPD rats(P＜0.05);Cell experiments showed that morin increased cell viability in COPD cell models and inhibited MMP9 ex-pression(P＜0.05).In addition,morin inhibited cell apoptosis and expression of inflammatory factors in the COPD cell model,but this effect was reversed by overexpression of MMP9(P＜0.05).Conclusion Morin can improve apoptosis and and expression of inflammatory cytokines in COPD cell model,reduce lung tissue pathologi-cal changes by inhibiting MMP9 expression.
