Characterization of persistent marine bacterial community profiles isolated from  long-term Kappaphycus alvarezii cultures in a closed cultivation system using 16S  rDNA analysis

Rennielyn Rupert; Kenneth Francis Rodrigues; Harry Lye Hin Chong; Nur Athirah Yusof; Wilson Thau Lym Yong

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Characterization of persistent marine bacterial community profiles isolated from long-term Kappaphycus alvarezii cultures in a closed cultivation system using 16S rDNA analysis

Author: Rennielyn Rupert ¹ ; Kenneth Francis Rodrigues ¹ ; Harry Lye Hin Chong ² ; Nur Athirah Yusof ¹ ; Wilson Thau Lym Yong ¹
Author Information

1. Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu, Sabah 88400, Malaysia.
2. Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu, Sabah 88400, Malaysia.
Publication Type:Journal Article
Keywords: 16S rDNA gene sequencing; closed cultivation system; Kappaphycus alvarezii; persistent marine bacteria; red algae
From:Malaysian Journal of Microbiology 2023;19(no.6):693-704
CountryMalaysia
Language:English
Abstract: Aims:The study aims to investigate the bacterial community profiles on the surface of red algae (Kappaphycus alvarezii) and persistent bacteria that can adapt to long-term cultivation in a closed circulation system.
Methodology and results:Kappaphycus alvarezii explants were cultured in a controlled laboratory setting for 30 days to investigate related bacterial adaptability to controlled culture conditions. Bacterial isolates associated with seedlings were subjected to 16S rDNA amplification and sequencing, followed by the construction of a phylogenetic tree using MEGA X software. The results show distinct microbial composition between the first and 30th days. The derived phylogenetic tree features three dominant phyla: Proteobacteria (Vibrio and Thalassospira), Pseudomonadota (Pseudoalteromonas, Alteromonas, Grimontia, Ruegeria, Phaebacter and Bacterioplanes) and Firmicutes (Bacillus). A comparative examination of these two bacterial groups (day 1 and day 30) reveals evidence of persistent marine bacteria, such as the genera Vibrio, Pseudoalteromonas, Alteromonas, Phaaebacter and Bacterioplanes, that successfully adapt to long-term cultivation within closed circulation systems.
Conclusion, significance and impact of study:The findings of this study contribute to the understanding of bacterial ecology in the controlled red algae cultivation environment and also provide valuable insights into the optimization of an ideal closed cultivation system for sustainable K. alvarezii production, benefiting the seaweed industry.
Full text:20.2023my0074.pdf