TMZ@ZIF-8 nanoparticles：preparation，characterization，and in vivo and in vitro anti-tumor studies
- VernacularTitle:TMZ@ZIF-8纳米粒的制备、表征及体内外抗肿瘤研究
- Author:
Ting LIU
1
,
2
;
Yi WANG
3
;
Binghui LI
1
;
Shuo HE
1
;
Jiang XIA
1
;
Ting ZHU
4
Author Information
1. School of Pharmacy，Harbin University of Commerce，Harbin 150076，China
2. College of Pharmacy，Harbin Medical University，Heilongjiang Daqing 163319，China
3. Honors’School，Harbin University of Commerce，Harbin 150028，China
4. Center of Pharmaceutical Engineering and Technology，College of Pharmacy，Harbin University of Commerce，Harbin 150076，China
- Publication Type:Journal Article
- Keywords:
temozolomide;
zeolite imidazole framework;
pH-sensitive;
glioma;
drug delivery system;
nanoparticle
- From:
China Pharmacy
2024;35(9):1100-1107
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To prepare zeolite imidazole framework （ZIF）-8 nanoparticles （NPs） loaded with temozolomide （TMZ） （abbreviated as TMZ@ZIF-8 NPs） drug delivery system， thus increasing drug enrichment and anti-glioma effects in lesions. METHODS After preparing ZIF-8 NPs using the room temperature solution reaction method， the impregnation method was used to prepare TMZ@ZIF-8 NPs drug delivery system. Characterization was carried out using transmission electron microscopy， laser particle size， and Fourier transform infrared spectroscopy， and dissolution and anti-tumor activity experiments in vitro and in vivo were conducted. RESULTS TMZ@ZIF-8 NPs were successfully prepared with the particle size of （126.23±7.92） nm， drug loading amount of （28.79±1.26）%， and 72 h cumulative dissolution rate of （72.36±3.62）%. The results of in vitro anti-tumor activity experiments showed that the relative cell survival rate of ZIF-8 NPs remained above 90%； the prepared TMZ@ZIF-8 NPs delivery system exhibited superior inhibition， higher uptake capacity， and better promoting apoptosis effects on the growth and proliferation of C6 cells as compared with the free TMZ. The results of in vivo anti-tumor activity experiments showed that ZIF-8 NPs were not enriched in the brain of rats， and the enrichment effect of TMZ in the brain was not significant， while TMZ@ZIF-8 NPs had a significant enrichment effect in the brain. CONCLUSIONS ZIF-8 NPs can effectively load TMZ， and successfully prepared TMZ@ZIF-8 NPs can improve TMZ uptake ability and anti-glioma effect.
