Establishment of UPLC characteristic chromatogram and component analysis of the volatile oil in the standard decoction of Qingshang juantong decoction
- VernacularTitle:清上蠲痛汤标准汤剂挥发油的UPLC特征图谱建立及成分分析
- Author:
Zhiying FAN
1
;
Qianqian ZHU
2
;
Xiehe WANG
2
;
Yanjuan ZHAI
2
;
Huimin WANG
2
;
Yangxin GU
2
;
Haiqin ZHOU
2
;
Tulin LU
1
;
Kewei ZHANG
1
;
Song LI
2
Author Information
1. School of Pharmacy，Nanjing University of Chinese Medicine，Nanjing 210023，China
2. Jiangyin Tianjiang Pharmaceutical Co.，Ltd.，Jiangsu Jiangyin 214434，China
- Publication Type:Journal Article
- Keywords:
Qingshang juantong decoction;
classic formula;
volatile oil;
characteristic chromatogram;
chemical constituents
- From:
China Pharmacy
2024;35(9):1082-1086
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish the characteristic chromatogram of the volatile oil in the standard decoction of Qingshang juantong decoction， and preliminarily infer the main active components of volatile oil that affect the clinical therapeutic effect. METHODS The volatile oil in the standard decoction of Qingshang juantong decoction was extracted by steam distillation. The ultra-high performance liquid chromatography （UPLC） characteristic chromatograms of 15 batches of samples were established by the Similarity Evaluation System of TCM Chromatographic Fingerprint （2012 edition）， and the similarity evaluation was carried out. The volatile oil of standard decoction was identified by UPLC coupled with quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF/MS）. Then the volatile oil components were analyzed by GC-MS. RESULTS The similarities of UPLC characteristic chromatograms for volatile oil of 15 batches of Qingshang juantong decoction were between 0.949 and 0.997. A total of 12 common peaks were obtained. According to the UPLC-Q-TOF/MS， the main components were methyl eugenol， E-ligustilide， E-butylidenephthalide and so on. A total of 23 components were identified by GC-MS， which were mainly 3，4，5-trimethoxy- methylbenzene， patchouli alcohol， Z-ligustilide and so on. CONCLUSIONS The characteristic chromatograms of the volatile oil in the standard decoction of Qingshang juantong decoction is established， and it is inferred that methyl eugenol， ligustilide， E- butylidenephthalide， patchouli alcohol， 3，4，5-trimethoxy-methylbenzene might be the main active components affecting the clinical therapeutic effect of the volatile oil of Qingshang juantong decoction.
