Study on original identification of Rhei Radix et Rhizoma decoction pieces based on electronic sensory system and GC-IMS technology
- VernacularTitle:基于电子感官系统和GC-IMS技术的大黄饮片基原辨识研究
- Author:
Shuo YANG
1
;
Zhongli XU
2
;
Xinzhi ZHAO
3
;
Dianhua SHI
3
,
4
;
Yanpeng DAI
3
,
4
;
Yu BI
1
;
Yizhou XIN
5
Author Information
1. School of Pharmacy，Shandong University of Traditional Chinese Medicine，Jinan 250014，China
2. Pingyi County Hospital of Traditional Chinese Medicine of Shandong Province，Shandong Pingyi 273300，China
3. Shandong Academy of Chinese Medicine，Jinan 250014，China
4. The Key Unit for Research of Technique and Principle of Honey-processing and Carbonizing of SATCM，Jinan 250014，China
5. Dept. of Pharmacy，the Affiliated Hospital of Shandong University of Traditional Chinese Medicine，Jinan 250014，China
- Publication Type:Journal Article
- Keywords:
Rhei Radix et Rhizoma decoction pieces
- From:
China Pharmacy
2024;35(9):1076-1081
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the variations in taste， aroma and volatile organic compounds of Rhei Radix et Rhizoma decoction pieces derived from different sources， and to identify their origins. METHODS The flavor， odor and volatile organic compounds of Rhei Radix et Rhizoma decoction pieces from different sources were compared and analyzed by using electronic tongue， electronic nose， and gas chromatography-ion mobility spectrometry （GC-IMS）. Principal component analysis （PCA）， partial least squares-discriminant analysis （PLS-DA）， orthogonal partial least squares discriminant analysis （OPLS-DA） and Fisher discriminant analysis were employed to identify the origins of Rhei Radix et Rhizoma decoction pieces and establish the basis discrimination criteria. RESULTS The differences in taste of Rhei Radix et Rhizoma decoction pieces from 3 origins were primarily characterized by bitterness， astringency， and bitter-astringent aftertaste. In terms of smell， variations were mainly observed in inorganic sulfides， organic sulfides containing aromatic components， methane and other short-chain alkanes， alcohols， ethers， aldehydes and ketones， as well as nitrogen oxides. Differentially volatile organic compounds mainly consisted of alcohols， aldehydes and ketones. Furthermore， the samples from 8 batches could be effectively classified into 3 categories.Three types of Rhei Radix et Rhizoma decoction pieces can be effectivily identified based on the peak intensity ratio between volatile substances. For example， when the peak intensity of 2-acetylfuran was 3-19 times that of isobutyric acid [dimer]， it was considered as Rheum officinale Baill. CONCLUSIONS The discriminant models established in this study， along with the criteria for determining the origins based on the peak intensity ofcharacteristic volatile compounds， can be utilized for the identification of Rhei Radix et Rhizoma decoction pieces.
