3, 3-Dimethyl-1-butanol attenuates ulcerative colitis and secondary liver injury by reducing trimethylamine production
10.11665/j.issn.1000-5048.2023080801
- VernacularTitle:3, 3-二甲基-1-丁醇抑制肠道三甲胺生成可缓解溃疡性结肠炎及继发性肝损伤
- Author:
Xinnan WANG
1
;
Lifang LIU
Author Information
1. 中国药科大学中药学院生药学系
- Publication Type:Journal Article
- Keywords:
choline metabolism / colitis / gut microbiota metabolism / trimethylamine
- From:
Journal of China Pharmaceutical University
2024;55(2):246-256
- CountryChina
- Language:Chinese
-
Abstract:
Abstract: Ulcerative colitis (UC) is a chronic intestinal disease caused by a variety of factors. Severe intestinal inflammation can also cause liver injury. Based on the previous research, microbial dysbiosis in the inflammatory state leads to the conversion of excess choline into trimethylamine (TMA) by the intestinal flora, which competes with the host for the use of the nutrient choline, and induces liver injury. 3, 3-dimethyl-1-butanol (DMB), a structural analogue of choline, can reduce TMA levels from choline conversion. The aim of this study was to investigate the protective effect and possible mechanism of DMB on UC and secondary liver injury. Dextran sulfate sodium-induced acute colitis model in mice was established. The weight of mice, and collected serum, liver and intestinal contents after mice sacrifice were measured. The morphological changes of colon and liver were observed; liver function was detected with the kit of biochemical indexes; UHPLC-MS/MS was applied to detect changes in choline metabolism in vivo. The experimental results showed that DMB could attenuate body weight loss index, improve colonic inflammation, and reduce liver injury in UC mice. The detection of choline-related metabolites in serum, intestinal contents and liver showed that DMB could effectively inhibit the production of trimethylamine in the intestine, improve the availability of host choline, effectively alleviate colitis deterioration, and reduce liver damage caused by severe intestinal lesions.
