Bifidobacterium pseudocatenulatum ATCC 27919 exopolysaccharides induced  autophagy and apoptosis against endoplasmic reticulum stress in Caco-2 cells

Husna Zulkipli; Hifa Nazirah Mohammed Yaziz; Mohd Yusri Idorus; Siti Hamimah Sheikh Abdul Kadir; Maslinda Musa; Khalilah Abdul Khalil

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Bifidobacterium pseudocatenulatum ATCC 27919 exopolysaccharides induced autophagy and apoptosis against endoplasmic reticulum stress in Caco-2 cells

Author: Husna Zulkipli ¹ ; Hifa Nazirah Mohammed Yaziz ² ; Mohd Yusri Idorus ³ ; Siti Hamimah Sheikh Abdul Kadir ⁴ ; Maslinda Musa ² ; Khalilah Abdul Khalil ²
Author Information

1. Faculty of Applied Sciences, Universiti Teknologi MARA, Perak Branch, 35400, Tapah Road, Perak, Malaysia.
2. Department of Biomolecular Science, Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia.
3. Institute of Medical Molecular Biotechnology (IMMB), Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, 47000 Sungai Buloh, Selangor, Malaysia.
4. Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, 47000 Sungai Buloh, Selangor, Malaysia.
Publication Type:Journal Article
Keywords: Apoptosis; autophagy; Bifidobacterium pseudocatenulatum ATCC 27919; colorectal cancer; exopolysaccharides
From:Malaysian Journal of Microbiology 2023;19(no.5):545-559
CountryMalaysia
Language:English
Abstract: Aims:This study aimed to investigate the effect of exopolysaccharide (EPS) from Bifidobacterium pseudocatenulatum ATCC 27919 in Caco-2 cells on apoptotic and autophagic pathways.
Methodology and results:Cell viability was examined by MTS assay and it showed a significant decrease in Caco-2 cells after exposure to EPS. Microscopy imaging and morphological analyses demonstrated that EPS-exposed Caco-2 cells exhibited the main morphological characteristics of apoptosis. EPS-exposed cells showed early apoptosis and cell cycle arrest at the G2/M phase in the cell cycle and dead cell assays. qPCR suggested that mRNA expression of substantial apoptotic markers such as cleaved Caspase-3, BAX and PARP-1 were significantly increased in EPS-exposed cells. The autophagy event was demonstrated in EPS-exposed cells by the contrary mRNA expression of Beclin-1 and Bcl-2 and the detection of autophagic LC3-II protein at 24 h exposure. GRP78 mRNA expression was also increased in EPS-exposed cells, indicating the occurrence of endoplasmic reticulum (ER) stress.
Conclusion, significance and impact of study :Autophagy activity in EPS-exposed Caco-2 cells preceded apoptosis, suggesting it was a cytoprotective response against ER stress. Research findings set the foundation for therapeutic CRC treatments and provide insight into its regulatory processes.
Full text:20.2023my0058.pdf