Anti-MDR bacterial activity of bacteria associated with sea sponge of Amphimedon  sp. from Karimunjawa Island, Central Java, Indonesia

Muhammad Evy Prastiyanto; Endah Retnaningrum; Sri Darmawati; Muhammad Ziddan Bayu Aji; Desty Ratna Putri; Afifah Khairunnisa; Nadia Yusraini Arilya; Keke Putri Wulansari; Umi Hanik Qoni'; ah

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Anti-MDR bacterial activity of bacteria associated with sea sponge of Amphimedon sp. from Karimunjawa Island, Central Java, Indonesia

Author: Muhammad Evy Prastiyanto ¹ ; Endah Retnaningrum ^{2
,

3} ; Sri Darmawati ^{4
,

5} ; Muhammad Ziddan Bayu Aji ⁵ ; Desty Ratna Putri ⁵ ; Afifah Khairunnisa ⁵ ; Nadia Yusraini Arilya ⁶ ; Keke Putri Wulansari ⁶ ; Umi Hanik Qoni'ah ⁶
Author Information

1. Microbiology Laboratory, Department of Medical Laboratory Technology, Faculty of Nursing and Health Sciences, Universitas Muhammadiyah Semarang. Jl. Kedungmundu Raya No. 18, Semarang50273, Central Java, Indonesia.
2. Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, Yogyakarta, 55281, Indonesia.&
3. Indonesia Natural Dye Institute (INDI), LPPT, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
4. Molecular Biology Laboratory, Department of Medical Laboratory Technology, Faculty of Nursing and Health Sciences, Universitas Muhammadiyah Semarang. Jl. Kedungmundu Raya No. 18, Semarang 50273, Central Java, Indonesia.&
5. Master Program of Clinical Laboratory Science, Universitas Muhammadiyah Semarang. Jl. Kedungmundu Raya No. 18, Semarang 50273, Central Java, Indonesia.
6. Division of Medical Laboratory Technology, Faculty of Nursing and Health Sciences, Universitas Muhammadiyah Semarang. Jl. Kedungmundu Raya No. 18, Semarang50273, Central Java, Indonesia.
Publication Type:Journal Article
Keywords: Antibacterial activity; Amphimedon sp.; Bacillus; MDR bacteria; sponge; wounds
From:Malaysian Journal of Microbiology 2023;19(no.5):518-526
CountryMalaysia
Language:English
Abstract: Aims:The aim of this study was to evaluate the antibacterial activity of bacteria associated with sea sponge Amphimedon sp. against multi-drug resistant (MDR) bacteria that cause wound infections.
Methodology and results :The antibacterial activity was evaluated by the overlay method. Identification of active bacterial symbionts was carried out using the 16S rRNA gene sequence-based method of bacterial identification. The results suggest that one of nine isolates had antibacterial activity against MDR bacteria. Isolate Z9VIII was identified as Bacillus subtilis and demonstrated robust antibacterial activity against bacteria: MRSA (11 mm), MDR-AB (17 mm) and CRPA (12 mm).
Conclusion, significance and impact of study:This study concludes that the one of the bacterial species associated with the sea sponge of Amphimedon sp. was B. subtilis, which has the potential as antibacterial agent against MDR bacteria.
Full text:20.2023my0055.pdf