Genomic characteristics analysis of a colistin and tigecycline-resistant Klebsiella pneumoniae
10.3969/j.issn.1006-2483.2024.03.009
- VernacularTitle:1株多黏菌素和替加环素耐药的肺炎克雷伯菌基因组特征分析
- Author:
Xinjing JIA
1
,
2
,
3
;
Xinran GONG
1
,
2
,
3
;
Peng LI
1
,
2
,
3
;
Chuanyuan DUAN
1
,
2
,
3
;
Lisha LIU
2
,
3
,
4
;
Dayang ZOU
1
,
2
,
3
;
Yong WANG
1
,
2
,
3
Author Information
1. Public Health School of China Medical University , Shenyang , Liaoning 110122 ,China
2. Center for Disease Control and Prevention of Chinese People'
3. s Liberation Army , Beijing 100071 , China
4. Public Health School of Anhui Medical University , Hefei , Anhui 230000 , China
- Publication Type:Journal Article
- Keywords:
Colistin;
Tigecycline;
Genome-wide characteristics;
Plasmid
- From:
Journal of Public Health and Preventive Medicine
2024;35(3):37-41
- CountryChina
- Language:Chinese
-
Abstract:
Objective In this study, a strain of colistin and tigecycline-resistant bacteria isolated in 2009 was analyzed, and the structure of drug-resistant plasmid and genetic environment were discussed, so as to provide basis for the prevention and control of multidrug-resistant bacteria. Methods A strain (GZ12244) with positive mcr and tet(M) was obtained by screening colistin and tigecycline resistance genes. Vitek-2 was used for strain identification, and the drug sensitivity test was carried out by broth dilution method. The molecular typing, drug resistance genes, insertion sequences, plasmid structure and genetic background were analyzed by genome-wide sequencing and bioinformatics. Results Strain GZ12244 is Klebsiella pneumoniae, which is resistant to colistin B, tigecycline, cefuroxime and tetracycline, and carries a variety of drug-resistant related genes such as mcr-1 and tet(M), and some of the drug-resistant genes with antibiotic efflux and antibiotic target change have amino acid substitution mutations. Mcr-1 and tet(M) coexist in a plasmid, and mcr-1 flanked by two insertion sequences ISApl1. There are insertion sequences such as IS15, IS1D and ISEc63 in the upstream and downstream of tet(M) gene. Conclusion Klebsiella pneumoniae GZ12244 is a multidrug-resistant strain. The drug-resistant gene exists in plasmid, and the mobile elements in upstream and downstream may spread the drug-resistant gene.
