Isolation of Enterobacteriaceae strains carrying mcr-1 resistance gene from Shanghai wastewater treatment plants and quantification of their copy number
10.19428/j.cnki.sjpm.2024.23481
- VernacularTitle:上海市污水处理厂携带mcr-1耐药基因肠道杆菌菌株的分离及拷贝数定量分析
- Author:
Jun FENG
1
;
Mingxiang LIU
2
;
Yuan ZHUANG
1
;
Miao PAN
1
;
Qian LIU
1
;
Yong CHEN
1
;
Jiayuan LUO
1
;
Jiayi FEI
1
;
Yitong WU
1
;
Yanqi ZHU
1
;
Jing ZHANG
2
;
Min CHEN
1
Author Information
1. Institute of Microbiology Laboratory， Shanghai Municipal Center for Disease Control and Prevention， Shanghai 200336， China
2. Hongkou District Center for Disease Control and Prevention， Shanghai 200082， China
- Publication Type:Journal Article
- Keywords:
wastewater treatment plant;
mcr⁃1 gene;
isolation;
copy number;
drug resistance;
Escherichia coli
- From:
Shanghai Journal of Preventive Medicine
2024;36(3):217-223
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo provide technical support for the molecular surveillance of pathogenic bacteria strains carrying mobile colistin resistance-1 （mcr⁃1） gene isolate from inlet of wastewater treatment plants （WWTP）. MethodsThe Enterobacteriaceae strains carrying mcr⁃1 resistance gene isolate from inlet of WWTP during April 1 to June 30， 2023 in Shanghai were cultured on blood-rich and SS culture medium and were identified using a mass spectrometry analyzer. The mcr⁃1 gene and copy number were detected by real-time fluorescence quantitative PCR. Drug susceptibility test was performed by microbroth dilution method. The copy numbers of Escherichia coli carrying mcr⁃1 gene isolated from wastewater and human fecel were statistically analyzed by SPSS 25.0. ResultsA total of 14 strains carrying the mcr⁃1 gene were isolated from 49 WWTP samples， and the positive isolation rate was 28.6%， including 12 non-diarrheal E. coli strains and 2 Klebsiella pneumoniae strains. The drug susceptibility results showed that all 14 strains were multi-drug resistant bacteria. They were all sensitive to imipenem and tigecycline， but were ampicillin- and cefazolin-resistant. There was no significant difference in the copy number between human-sourced diarrheal E. coli and wastewater-sourced non-diarrheal E. coli （t=0.647， P>0.05）. ConclusionThe isolation and identification of strains carrying the mcr⁃1 gene from inlet of WWTP samples were firstly established in Shanghai. The multi-drug resistance among the isolated strains is severe. To effectively prevent and control the spread of colistin-resistant bacteria， more attention should be paid to the surveillance of mcr⁃1 gene.
