Virulence-associated traits and antimicrobial resistance of the uropathogenic Escherichia coli (UPEC) strains in relation to phylogenetic background and host factors from Malaysian patients
http://dx.doi.org/10.21161/mjm.220134
- Author:
Jia-Jin Chin
1
;
Quok-Cheong Choo
2
;
Murnihayati Hassan
3
;
Wai-Yew Ho
4
;
Choy-Hoong Chew
1
Author Information
1. Department of Allied Health Sciences, Faculty of Science, Universiti Tunku Abdul Rahman (UTAR), Kampar, 31900 Perak, Malaysia.
2. Department of Biological Science, Faculty of Science, Universiti Tunku Abdul Rahman (UTAR), Kampar, 31900 Perak, Malaysia.
3. Bacteriology Unit, Infectious Disease Research Center (IDRC), Institute for Medical Research (IMR), National Institutes of Health (NIH), Setia Alam, 40170 Shah Alam, Selangor, Malaysia.
4. Centre for Foundation Studies, Universiti Tunku Abdul Rahman, Kampar, 31900 Perak, Malaysia.
- Publication Type:Journal Article
- Keywords:
Age;
multidrug resistance;
urinary tract infection;
uropathogenic Escherichia coli;
virulence traits
- From:Malaysian Journal of Microbiology
2023;19(no.2):211-221
- CountryMalaysia
- Language:English
-
Abstract:
Aims: Hundreds of uropathogenic Escherichia coli (UPEC) lineages with diverse virulence-associated traits have been reported worldwide. However, the complex interactions between the bacterial traits and host factors remain largely unexplored. This study was aimed to determine the distribution of virulence-associated traits, phylogenetic background and antimicrobial resistance profiles of the UPEC isolates in relation to the host factors.
Methodology and results:Polymerase chain reactions (PCRs) were conducted to determine the prevalence of 32 virulence genes (VGs), eight pathogenicity island (PAI) markers and phylogroups of 105 UPEC isolates. The
antimicrobial susceptibility testing was performed using the disc diffusion method. Results suggest that the virulenceassociated traits and antimicrobial resistance profiles of the UPEC strains were associated with phylogenetic background, host age and gender. Most of the virulence-associated traits tested were distributed prevalently in phylogroup B2, age group 40-59 and male gender, including papC, papG II_III, papG allele III, sfa/focDE, yfcV, hlyA, cnf1, cdtB, malX, PAI I536, PAI II536, PAI ICFT073, PAI IICFT073 and PAI IIJ96. Besides, higher rates of multidrug resistance (MDR) were significantly associated with non-phylogroup B2 (25/38; 65.8%; p<0.05) and age group 60-79 (29/42;
69.0%; p<0.05) but not with male gender (14/25; 56.0%; p=0.527). The UPEC strains collected from males and age 60-79 were significantly resistant to cefuroxime, cefotaxime and ceftazidime (all p<0.05).
Conclusion, significance and impact of study:Research findings elucidate the key molecular characteristics of the UPEC strains in relation to the host age and gender.
- Full text:20.2023my0024.pdf
