Structural Characteristics and Antioxidant Activity Analysis of Polysaccharides from Pinelliae Rhizoma and Its Processed Products Before and After Hydrolysis （Enzymolysis） by Sugar Spectrum
10.13422/j.cnki.syfjx.20240461
- VernacularTitle:基于糖谱法的半夏及其炮制品多糖结构特征分析及水/酶解前后抗氧化活性评价
- Author:
Meibian HU
1
;
Kuixu GAO
1
;
Yao WANG
1
;
Xi PENG
1
;
Jingya WANG
1
;
Xianglong MENG
1
;
Shuosheng ZHANG
1
;
Jianghua LI
2
;
Yujie LIU
1
Author Information
1. School of Traditional Chinese Medicine（TCM） and Food Engineering，Shanxi Provincial Key Laboratory of TCM Processing，Shanxi University of Chinese Medicine，Jinzhong 030619，China
2. School of Pharmacy，Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province，Chengdu University，Chengdu 610106，China
- Publication Type:Journal Article
- Keywords:
Pinelliae Rhizoma;
processed products;
polysaccharides;
glycosidases;
saccharide mapping;
antioxidant;
high performance thin layer chromatography（HPTLC）;
fluorescence-assisted carbohydrate gel electrophoresis（PACE）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(11):192-201
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThe glycosidic linkage structural characteristics of polysaccharides from Pinelliae Rhizoma（PR） and its processed products were analyzed by sugar spectrum， high performance thin layer chromatography（HPTLC）， fluorescence-assisted carbohydrate gel electrophoresis（PACE） based on partial acid hydrolysis and specific glycosidase hydrolysis， and the antioxidant activities of polysaccharides before and after hydrolysis（enzymolysis） were compared. MethodPolysaccharides from PR and its processed products were extracted by ultrasound extraction， starch was hydrolyzed by α-amylase， and small molecules below 3 kDa were removed by ultrafiltration. The purified polysaccharides were prepared by hydrolysis of acid and five different specific glycosidases， and the hydrolysates were analyzed by HPTLC and PACE. The antioxidant capacity of polysaccharides was analyzed by 2，2′-azino-bis（3-ethylbenzthiazoline-6-sulfonic acid）（ABTS） and 2，2-diphenyl-1-picrylhydrazyl（DPPH） free radical scavenging experiment before and after different hydrolysis. ResultThrough HPTLC and PACE analysis， it was found that polysaccharides from PR and its processed products could be hydrolyzed by β-galactosidase， β-mannase， cellulase and pectinase， but hardly hydrolyzed by glucanase， indicating that the polysaccharides contained β-galactopyranoside bond， β-1，4-mannoside bond， β-1，4-glucoside bond and α-1，4-galacturonic acid glycosidic bond. In vitro antioxidant experiments showed that the ABTS radical scavenging capacity of the polysaccharides from PR and its processed products was weakened after acid hydrolysis and pectinase enzymatic hydrolysis， while the ABTS radical scavenging capacity was enhanced after enzymatic hydrolysis with cellulase， β-galactosidase， and β-mannase. And after different hydrolysis， the DPPH free radical scavenging capacity of polysaccharides from PR and its processed products was all significantly enhanced. ConclusionThe glycosidic linkage structural characteristics of polysaccharides from PR and its processed products was analyzed by sugar spectrum in this paper， and the relationship between glycosidic bond types and their antioxidant activity was clarified through in vitro antioxidant experiments， which is beneficial for further elucidating the material basis of the related efficacy of PR and its processed products， and providing new ideas and methods for analyzing the structural characteristics of polysaccharides in Chinese medicines.
