Effect and Mechanism of Water Extract of Mori Folium on Oxidative Stress in Adipose Tissue of T2DM Mice
10.13422/j.cnki.syfjx.20240428
- VernacularTitle:桑叶水提物对2型糖尿病小鼠皮下脂肪组织氧化应激的影响及机制
- Author:
Huilin ZHANG
1
;
Yongcheng AN
1
;
Changhao HE
1
;
Yan HUANG
2
;
Wanxin FU
2
;
Menglu WANG
1
;
Ziyi SHAN
2
;
Yuhang DU
1
;
Jiamei XIE
1
;
Zhanhong JIA
1
;
Baosheng ZHAO
3
Author Information
1. College of Chinese Materia Medica， Beijing University of Chinese Medicine， Beijing 102488， China
2. College of Life Sciences， Beijing University of Chinese Medicine， Beijing 102488， China
3. Beijing Research Institute of Chinese Medicine， Beijing University of Chinese Medicine， Beijing 100029， China
- Publication Type:Journal Article
- Keywords:
water extract of Mori Folium;
type 2 diabetes mellitus;
oxidative stress;
adipose tissue
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(11):43-50
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of water extract of Mori Folium （MLE） on oxidative stress in adipose tissue of type 2 diabetes mellitus （T2DM） mice and explore its mechanism. MethodTwenty-four male db/db mice were randomly divided into model group， metformin group， low-dose MLE （MLE-L） group， and high-dose MLE （MLE-H） group according to their body weight and blood glucose， with six mice in each group， and other six C57BLKS/JGpt wild littermate mice were selected as normal group. The mice in the metformin group were given 200 mg·kg-1 metformin suspension， and the mice in the MLE-L and MLE-H groups were respectively given 2 g·kg-1 and 4 g·kg-1 MLE， while the mice in the normal group and model group were given the same dose of deionized water by daily gavage for eight weeks. Body weight， subcutaneous fat index， fasting blood glucose （FBG）， and oral glucose tolerance level （OGTT） of the mice were detected， and serum superoxide dismutase （SOD）， glutathione peroxidase （GSH-Px）， and malondialdehyde （MDA） were measured. The expression levels of silent information regulator 1 （SIRT1） and NADPH oxidase type 4 （NOX4） protein in subcutaneous adipose tissue of the mice were detected by Western blot. ResultThe FBG level， OGTT， and subcutaneous fat index of T2DM mice were significantly decreased （P<0.05， P<0.01） after administration of MLE compared with the blank group. The contents of serum SOD and GSH were significantly increased， while the level of oxidative stress damage marker MDA was significantly decreased （P<0.05， P<0.01）. The expression of SIRT1 protein in adipose tissue was significantly increased， while the expression of NOX4 protein was significantly decreased （P<0.05， P<0.01）. ConclusionMLE can ameliorate T2DM by alleviating oxidative stress in adipose tissue of T2DM mice and reducing blood glucose.
