Regulatory effect of autophagy on the resistance of human liver cancer cell Huh7 to lenvatinib
- VernacularTitle:自噬在人肝癌细胞Huh7对仑伐替尼耐药中的调节作用
- Author:
Dahong CHEN
1
;
Yafei WU
1
;
Wenjing DIAO
1
;
Huihua YANG
1
;
Pengjuan MAO
1
;
Qin LI
1
Author Information
1. Dept. of Clinical Pharmacy，Shanghai General Hospital，Shanghai Jiao Tong University School of Medicine，Shanghai 200080，China
- Publication Type:Journal Article
- Keywords:
autophagy;
lenvatinib;
liver cancer cell;
drug resistance
- From:
China Pharmacy
2024;35(8):961-966
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the regulatory effect of autophagy on the resistance of human liver cancer cell Huh7 to lenvatinib. METHODS Using human liver cancer cell Huh7 as subject， the lenvatinib-resist cell model （Huh7-LR） was generated by the low-dose gradient method combined with long-term administration. The sensitivity of parental cell Huh7 and drug-resistant cell Huh7-LR to lenvatinib was detected by using CCK-8 assay and flow cytometry. Western blot assay and GFP-mCherry-LC3 plasmid transfection were performed to detect the expression levels of autophagic protein Beclin-1， autophagic adapter protein sequestosome 1 （p62）， microtubule-associated protein 1 light chain 3 （LC3） and autophagic level. Furthermore， an autophagy activation model was constructed by cell starvation， the protein expression of p62 and autophagy level were detected by using Western blot assay and GFP-mCherry-LC3 plasmid transfection， and the effect of autophagy activation on the sensitivity of Huh7-LR cells to lenvatinib was detected by flow cytometry. RESULTS Compared with parental cells， the drug resistance index of Huh7-LR cells was 6.2； protein expression of p62 was increased significantly， while apoptotic rate， protein expression of Beclin-1 and LC3Ⅱ/ LC3Ⅰ ratio were all reduced significantly （P＜0.05 or P＜0.01）； the level of autophagy was decreased to some extent. Autophagy activation could significantly increase the protein expression of p62 in Huh7-LR cells （P＜0.05） and autophagy level， and significantly increase its apoptotic rate （P＜0.05）. CONCLUSIONS Autophagy is involved in lenvatinib resistance， and activating autophagy can reverse the resistance of liver cancer cells to lenvatinib to some extent.
