The characteristics of bone disseminated cells of 4T1
10.16438/j.0513-4870.2023-1178
- VernacularTitle:4T1细胞的骨播散型细胞的特征研究
- Author:
Zhuo WANG
1
;
Xian-jing LI
2
;
Yan LI
1
Author Information
1. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
2. State Key Laboratory of Natural Medicine, China Pharmaceutical University, Nanjing 211198, China
- Publication Type:Research Article
- Keywords:
breast cancer;
bone metastasis;
isseminated breast tumor cell;
epithelial mesenchymal transition;
fatty acid metabolism
- From:
Acta Pharmaceutica Sinica
2024;59(3):608-615
- CountryChina
- Language:Chinese
-
Abstract:
Based on bone metastasis potential of mouse breast cancer 4T1 cells, the bone disseminated breast tumor cells 4T1 (B-4T1) were acquired through the screening of 6-mercaptopurine. The characteristics of B-4T1 were studied by morphological observation, proliferation assay, expression of epithelial and mesenchymal cell markers detection, transcriptome sequencing, and tumor formation experiments. The results showed that B-4T1 was round and spindle-shaped than primary 4T1 cells, and its proliferation rate was reduced, as well as epithelial cell adhesion molecule (EpCAM) and E-cadherin expression. The transcript level of N-cadherin was increased in the B-4T1, but not vimentin, indicating that B-4T1 had partial epithelial mesenchymal transition. Besides, B-4T1 had higher fatty acid metabolism and better tumor formation capacity. This study lays the experimental foundation for the basic study of metastasis in breast cancer. All animal experiments in this paper were conducted in accordance with the standards of the Animal Ethics Committee of China Pharmaceutical University.
