Based on LC-MS technology explored the metabolomics of Agrimonia pilosa intervening in non-small cell lung cancer A549 cells
10.16438/j.0513-4870.2023-0777
- VernacularTitle:基于LC-MS技术探讨仙鹤草干预非小细胞肺癌A549细胞的代谢组学研究
- Author:
Ze-hua TONG
1
;
Wen-jun GUO
1
;
Han-rui ZOU
1
;
Li-wei XU
2
;
Ya-juan XU
3
;
Wei-fang WANG
1
Author Information
1. Changchun University of Chinese Medicine, Changchun 130117, China
2. Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130021, China
3. Jilin Academy of Chinese Medicine Sciences, Changchun 130012, China
- Publication Type:Research Article
- Keywords:
italic>Agrimonia pilosa;
metabolomics;
A549 cell;
apoptosis;
cell cycle
- From:
Acta Pharmaceutica Sinica
2024;58(3):704-712
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this study was to analyze the effects on cell viability, apoptosis, and cell cycle of non-small cell lung cancer (NSCLC) A549 cells after intervention with Agrimonia pilosa (AP) and investigate Agrimonia pilosa anti-tumor activity in vitro. Meanwhile, liquid chromatography mass spectrometry (LC-MS) metabolomics technology was used to analyze the changes of cellular metabolites and metabolic pathways. The results of this study will provide a theoretical and experimental basis for investigating the mechanism of the effect of Agrimonia pilosa on non-small cell lung cancer A549 cells. The results showed that the cell nucleus of A549 cells crumpled and apoptosis occurred with the increase of drug concentration. The survival rate of the cells decreased, and the inhibition rate reached 21.5% and 91.74% under the low and high dose conditions, respectively. Lactate dehydrogenase (LDH) content increased (P < 0.05). Metabolomics results showed significant differences in metabolism between groups, thirty-three distinct metabolites including LysoPC(24:0/0:0), LysoPC(17:0/0:0) and PC(O-40:5) were deduced. The pathway enrichment showed that the Agrimonia pilosa plays an anti-tumor role mainly by regulating the metabolism of glycerophosphate and purine in A549 cells, in which the effect on glycerophosphate metabolism pathway was most significant. The results of combined pharmacodynamics suggested that Agrimonia pilosa might induce apoptosis and inhibit the growth of A549 cells by regulating LysoPC(24:0/0:0), LysoPC(17:0/0:0) and PC(O-40:5) metabolites in A549 cells.
