Culture of rabbit corneal stromal cells on thermo-sensitive materials by the radiation polymerization
10.3980/j.issn.1672-5123.2024.5.05
- VernacularTitle:辐射合成的温敏材料上兔角膜基质细胞的培养
- Author:
Wei WANG
1
;
Pengfei ZHANG
1
;
Chunxia CHEN
1
;
Li QIAN
1
;
Jiansu CHEN
1
Author Information
1. Department of Ophthalmology, Tongling People's Hospital, Tongling 244000, Anhui Province, China
- Publication Type:Journal Article
- Keywords:
poly N-isopropylacry-lamide(PNIPAAm);
thermo-sensitive materials;
cell sheet;
corneal stroma;
cell culture
- From:
International Eye Science
2024;24(5):686-690
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the synthesis of thermo-sensitive poly N-isopropylacry-lamide(PNIPAAm)and the petri dish grafted with PNIPAAm hydrogels by the electron accelerator, as well as the growth conditions and the biological characteristics of rabbit corneal stromal cells on thermo-sensitive PNIPAAm hydrogels, and the cell sheets obtained from the PNIPAAm hydrogels.METHODS: NIPAAm monomer was dissolved in 2-propanol at concentrations of 55% with 0.5% N,N'-Methylenebisacry-lamide(MBA). Solution(70 μL)was added and spread uniformly over 35 mm petri dish. These dishes were immediately subjected to irradiation. After follow-up treatment, rabbit corneal stromal cells were cultured on thermo-sensitive petri dish in vitro.RESULTS: According to the monomer formula and radiation synthesis scheme in this experiment, PNIPAAm can be synthesized on the surface of the petri dish. Rabbit corneal stromal cells grew well in the thermo- sensitive surface and can be separated into sheets.CONCLUSION: The single and multilayer carrier-free cell sheets can be obtained from the use of thermo-sensitive petri dish.
