In Vivo MR Imaging of Magnetically Labeled Mesenchymal Stem Cells in a Rat Model of Renal Ischemia.
10.3348/kjr.2009.10.3.277
- Author:
Sung Il JUNG
1
;
Seung Hyup KIM
;
Hyo Cheol KIM
;
Kyu Ri SON
;
Se Young CHUNG
;
Woo Kyung MOON
;
Hoe Suk KIM
;
Jong Sun CHOI
;
Min Hoan MOON
;
Chang Kyu SUNG
Author Information
1. Department of Radiology, Konkuk University Medical Center, Research Institute of Medical Science, Konkuk University School of Medicine, Seoul 143-729, Korea. radsijung@kuh.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Mesenchymal stem cell;
Superparamagnetic iron oxide, MR;
Kidney, ischemia
- MeSH:
Animals;
Disease Models, Animal;
Ischemia/*diagnosis/*therapy;
Kidney/blood supply/pathology;
Kidney Diseases/*therapy;
Magnetic Resonance Imaging/*methods;
Male;
Mesenchymal Stem Cell Transplantation/*methods;
Mesenchymal Stem Cells/*pathology;
Rats;
Rats, Sprague-Dawley
- From:Korean Journal of Radiology
2009;10(3):277-284
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: This study was designed to evaluate in vivo MR imaging for the depiction of intraarterially injected superparamagnetic iron oxide (SPIO)-labeled mesenchymal stem cells (MSCs) in an experimental rat model of renal ischemia. MATERIALS AND METHODS: Left renal ischemia was induced in 12 male Sprague-Dawley rats by use of the catheter lodging method. In vivo MR signal intensity variations depicted on T2*-weighted sequences were evaluated in both the left and right kidneys prior to injection (n = 2), two hours (n = 4), 15 hours (n = 2), 30 hours (n = 2) and 72 hours (n = 2) after injection of SPIO-labeled MSCs in both kidneys. Signal intensity variations were correlated with the number of Prussian blue stain-positive cells as visualized in histological specimens. RESULTS: In an in vivo study, it was determined that there was a significant difference in signal intensity variation for both the left and right cortex (40.8 +/- 4.12 and 26.4 +/- 7.92, respectively) and for both the left and right medulla (23.2 +/- 3.32 and 15.2 +/- 3.31, respectively) until two hours after injection (p < 0.05). In addition, signal intensity variation in the left renal cortex was well correlated with the number of Prussian blue stain-positive cells per high power field (r = 0.98, p < 0.05). CONCLUSION: Intraarterial injected SPIO-labeled MSCs in an experimental rat model of renal ischemia can be detected with the use of in vivo MR imaging immediately after injection.
