Effect and Mechanism of Menispermi Rhizoma Total Alkaloids on Antiviral Infection
10.13422/j.cnki.syfjx.20240406
- VernacularTitle:北豆根总生物碱的抗病毒感染功能与机制
- Author:
Xuejiao WANG
1
;
Qiqi LI
2
;
Yanli YU
2
;
Xia LIU
1
;
Min LI
1
;
Zhe LIU
2
;
Xin JIA
2
;
Yao WANG
1
Author Information
1. School of Life Science，Beijing University of Chinese Medicine，Beijing 100027，China
2. School of Chinese Materia Medica，Beijing University of Chinese Medicine，Beijing 100027，China
- Publication Type:Journal Article
- Keywords:
Menispermi Rhizoma total alkaloids;
influenza A virus （H1N1）;
vesicular stomatitis virus （VSV）;
encephalomyocarditis virus （EMCV）;
antiviral immunity;
type Ⅰ interferon （IFN-Ⅰ）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(10):37-44
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the antiviral effect of Menispermi Rhizoma total alkaloids and its relationship with the type Ⅰ interferon （IFN-Ⅰ） signaling pathway. MethodThe effects of Menispermi Rhizoma total alkaloids on the intracellular replication of influenza A virus （H1N1）， vesicular stomatitis virus （VSV）， and cerebral myocarditis virus （EMCV） were detected by fluorescent inverted microscope， flow cytometry， Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR）， and Western blot. A mouse model infected with H1N1 was constructed， and the mice were divided into a control group， H1N1 model group， Menispermi Rhizoma total alkaloids groups （10， 20， 30 mg·kg-1）， and oseltamivir group （40 mg·kg-1）， so as to study the effects on the weight and survival rate of infected mice. Real-time PCR was used to detect the activation effect of Menispermi Rhizoma total alkaloids on the IFN-Ⅰ pathway in cells， and the relationship between the antiviral effect of Menispermi Rhizoma total alkaloids in IFNAR1 knockout A549 cells （IFNAR1-/--A549） and IFN-Ⅰ pathway was detected. ResultCompared with the control group， the virus proliferated significantly in the model group （P<0.01）. Compared with the model group， Menispermi Rhizoma total alkaloids could significantly inhibit the replication of H1N1， VSV， and EMCV in vitro （P<0.01）， inhibit the weight loss of the mice infected with the H1N1 in vivo， and improve the survival rate of mice （P<0.05）. In addition， Menispermi Rhizoma total alkaloids activated the IFN-I pathway and relied on this pathway to exert the function of antiviral infection. ConclusionMenispermi Rhizoma total alkaloids exert antiviral effects in vivo and in vitro by activating the IFN-Ⅰ pathway.
