Effect of Precocious Puberty on Glucose and Lipid Metabolism in Female Rats
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.20240305.005
- VernacularTitle:雌性大鼠性早熟对糖脂代谢的影响
- Author:
Xiaoya LIN
1
;
Baojiang HUANG
1
;
Jun ZHANG
1
;
Song GUO
1
;
Huamei MA
1
;
Yanhong LI
1
;
Minlian DU
1
;
Qiuli CHEN
1
Author Information
1. Department of Pediatrics， The First Affiliated Hospital of Sun Yat-sen University， Guangzhou 510080， China
- Publication Type:Journal Article
- Keywords:
precocious puberty;
glucose metabolism;
lipid metabolism;
insulin resistance;
female rat
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2024;45(2):233-242
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effect of precocious puberty on glucose metabolism and lipid metabolism in female rats. MethodsSixty two-day-old female rats were randomly divided into 2 groups. When aged 5 days， the precocious puberty group and normal group were given a single subcutaneous injection of danazol and solvent soybean oil respectively. The vaginal opening of rats was monitored from their 21 days of age. After 12 hours of fasting， all successful modeling rats were randomly executed within 3 days after vaginal opening， when aged 7 and 12 weeks. Then we measured the rats’ body weight and length， determined the concentrations of glucose， insulin， blood lipids， estradiol， leptin and adiponectin with enzyme-linked immunosorbent assay and observed the pathological changes of perirenal fat， uterus and ovary. ResultsFor body weight and length， rats in the precocious puberty group were smaller than those in the normal group within 3 days after vaginal opening， but which did not affect their subsequent growth and development， and there was no significant difference between the two groups at 7 and 12 weeks of age. Within 3 days after vaginal opening， insulin levels had significant difference between the two groups （P = 0.001）， the precocious group showed hyperinsulinemia and increased number of perirenal adipocytes. At three execution times， no significant difference was noted in estradiol， leptin and adiponectin levels between the two groups. The same was true in the ratios of ovary or uterus to body weight between the two groups. ConclusionsPrecocious puberty makes earlier onset of pubertal development and allows body maladaptation to the sudden changes of the internal environment. However， the changes due to precocious puberty are temporary and reversible， and they may become normal in adulthood.
